Immunocytochemical Methods and Protocols - Javois, Lorette C.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1995)
Overview of Antibody Use in Immunocytochemistry -- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration -- Purification of Antibodies Using Ion-Exchange Chromatography -- Purification of Antibodies Using Affinity Chromatography -- Purification of Antibodies Using Protein A-Sepharose and FPLC -- Conjugation of Fluorochromes to Antibodies -- Biotinylation of Antibodies -- Overview of Cell Fixation and Permeabilization -- Preparation of Frozen Sections for Analysis -- Processing of Cell-Touch Preparations -- Processing of Cytological Specimens -- Processing of Tissue-Culture Cells -- Processing of Tissue Specimens -- Overview of Fluorophores -- Direct Immunofluorescent Labeling of Cells -- Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts -- Overview of Antigen Detection Through Enzymatic Activity -- The Peroxidase-Antiperoxidase (PAP) Method -- The Avidin-Biotin Complex(ABC) Method -- Labeled Avidin Binding (LAB) Method* -- Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue -- ELISA on Attachment-Dependent or Suspension Grown Cells -- Use of Immunogold with Silver Enhancement -- Overview of Flow Cytometry and Fluorescent Probes for Cytometry -- Tissue Disaggregation -- Indirect Immunofluorescent Labeling of Viable Cells -- Indirect Immunofluorescent Labeling of Fixed Cells -- Fluorescent Labeling of DNA -- Deparaffinization and Processing of Pathologic Material -- Assay for Phagocytosis -- Assay for Filamentous Actin -- Assay for Chemoattractant Binding -- Assay for Oxidative Metabolism -- Fixation and Embedding -- Preparation of Colloidal Gold -- Conjugation of Colloidal Gold to Proteins -- Colloidal Gold/Streptavidin Methods -- Pre-Embedding Labeling Methods -- Postembedding Labeling Methods -- Overview of Fluorescence Photomicrography -- Overview of Fluorescence Analysis with the Confocal Microscope -- Overview of Laser Microbeam Applications as Related to Antibody Targeting -- Nucleic Acid Immunocytochemistry -- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes -- Overview of Automated Immunostainers -- Overview of Immunocytochemical Approaches to the Differential Diagnosis of Tumors.
Keywords: Immunology; Immunology