In Vitro Transcription and Translation Protocols - Tymms, Martin J.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1995)
Transcription In Vitro Using Bacteriophage RNA Polymerases -- Subtraction Hybridization cDNA Libraries -- Quantitative Measurement of mRNA Using the RNase Protection Assay -- Assembly and Transcription of Chromatin Templates Using RNA Polymerase III -- Antisense Affinity Depletion of RNP Particles -- In Vitro mRNA Editing Using S100 Extracts -- An In Vitro Transcription Assay for Probing Drug-DNA Interactions During Active Transcription of DNA -- In Vitro Reconstitution of Progesterone-Dependent RNA Transcription in Nuclear Extracts of Human Breast Carcinoma Cells -- Plastid In Vitro Transcription -- Microinjection of In Vitro Transcribed RNA and Antisense Oligonucleotides in Mouse Oocytes and Early Embryos to Study the Gain-and Loss-of-Function of Genes -- mRNA Translation in Xenopus Oocytes -- Transcription System Using a HeLa Cell Mitochondrial Lysate -- The Xenopus Egg Extract Translation System -- In Vitro Translation Using Rabbit Reticulocyte Lysate -- An In Vitro Transcription System from BmN Cells of the Silkworm, Bombyx mori -- Translation Using a Wheat-Germ Extract -- In Vitro Transcription and Translation in a Cell-Free System from Clostridium tetani -- Preparation and Use of E. coli S-30 Extracts -- Posttranslational Transport and In Thylakoido Integration of Plastid Protein -- Import into isolated yeast mitochondria of radiolabeled proteins synthesized in vitro -- Structure-Function studies based on in vitro expression -- ?-Like Plastid Transcription Factors -- Preparation of HeLa Nuclear Extracts -- Purification of the human TATA-Binding Protein, TBP -- DNA-Binding studies using in vitro synthesized myb proteins -- DNase i footprinting using PCR-generated end-labeled DNA probes -- Gene Isolation by Screening ?gt11 Expression Libraries with DNA Binding-Site Probes -- Transcriptional activation analysis by the Chloramphenicol Acetyl Transferase (CAT) enzyme assay.
Keywords: Cytology; Cell Biology