Monoclonal Antibody Protocols - Davis, William C.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1995)
Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies -- Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro -- Culture Conditions that Optimize Outgrowth of Hybridomas -- Production of Stable Heterohybridomas Producing Human Monoclonal Antibodies -- Production of Stable Bovine-Murine Interspecies Hybrids -- Production of Monoclonal Antibodies in Swine -- Production of Monoclonal Antibodies in Horses -- Use of Electric-Field-Mediated Cell Fusion to Produce Hybridomas Secreting Monoclonal Antibodies -- Rapid Isotyping of Mouse Monoclonal Antibodies -- Methods of Measuring Antibodies in Sera/Plasma, Ascites, and Tissue-Culture Media -- Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay -- Use of Monoclonal Antibodies in Immunohistochemistry -- Use of Monoclonal Antibodies in Immunoelectron Microscopy -- Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection -- The Use of Directly and Indirectly Labeled Monoclonal Antibodies in Flow Cytometry -- Use of Monoclonal Antibodies and Flow Cytometry to Cluster and Analyze Leukocyte Differentiation Molecules -- In Vivo and In Vitro Production of Monoclonal Antibodies -- Separation of Monoclonal Antibodies from Cell-Culture Supernatants and Ascites Fluid Using Thiophilic Agarose -- Detection, Purification, and Utilization of Murine Monoclonal IgM Antibodies -- Preparation and Use of Immunoaffinity Columns with Monoclonal Antibodies Without Purification from Ascites and Tissue-Culture Medium -- Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography -- Coupling of Monoclonal Antibodies with Fluorophores -- Coupling of Monoclonal Antibodies with Biotin -- Coupling of Monoclonal Antibodies with Enzymes -- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Negative Selection -- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Positive Selection.
Keywords: Immunology; Immunology