Plant Gene Transfer and Expression Protocols - Jones, Heddwyn; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1995)
Agrobacterium-Mediated Transformation -- Tools for Expressing Foreign Genes in Plants -- of Cloning Plasmids into Agrobacterium tumefaciens -- Leaf Disk Transformation Using Agrobacterium tumefaciens-Expression of Heterologous Genes in Tobacco -- Agobacterium rhizogenes as a Vector for Transforming Higher Plants -- Agrobacterium-Mediated Transformation of Arabidopsis thaliana -- Agrobacterium -Mediated Transfer of Geminiviruses to Plant Tissues -- Direct Gene Transfer -- Stable Transformation of Barley via Direct DNA Uptake -- Gene Transfer into Plant Protoplasts by Electroporation -- Transformation of Cereals by Microprojectile Bombardment of Immature Inflorescence and Scutellum Tissues -- Use of Reporter Genes -- The ?-Glucuronidase (gus) Reporter Gene System -- Chloramphenicol Acetyl Transferase Assay -- NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants -- Study of Gene Organization by Southern Blotting and Inverse PCR -- Gene Characterization by Southern Analysis -- Isolation and Characterization of Plant Genomic DNA Sequences via (Inverse) PCR Amplification -- RNA Techniques for Studying Gene Expression -- Isolation of Whole Cell (Total) RNA -- Poly(A)+RNA Isolation -- In Vitro Translation -- Northern Analysis and Nucleic Acid Probes -- Nuclear "Run-On" Transcription Assays -- RNase A/T1 Protection Assay -- Primer Extension Assay -- Applications of RT-PCR -- In Vitro Transcription of Class II Promoters in Higher Plants -- Analysis of Plant Gene Expression by Reverse Transcription-PCR -- In Situ Hybridization to Plant Tissue Sections -- Xenopus Oocytes as a Heterologous Expression System -- Heterologous Expression in Yeast -- Techniques for Studying Chloroplast Gene Expression -- The Isolation of Intact Chloroplasts -- In Vitro Protein Import by Isolated Chloroplasts -- Targeting of Foreign Proteins to the Chloroplast -- Techniques for Studying Mitochondrial Gene Expression -- Isolation of Mitochondria -- Mitochondrial Nucleic Acid Purification and Analysis -- In organello Protein Synthesis -- Immunological Detection of Proteins -- Separation of Plant Proteins by Electrophoresis -- Western Blotting Analysis -- ELISA Detection of Foreign Proteins -- Immunocytochemical Localization of Proteins.
Keywords: Cell biology; Cell Biology