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02287nam a22002895i 4500 |
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978-1-59259-541-9 |
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20190618101631.0 |
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cr nn 008mamaa |
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100301s1996 xxu| s |||| 0|eng d |
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|a 9781592595419
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| 024 |
7 |
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|a 10.1385/0896033139
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Markie, David.
|e author.
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| 245 |
1 |
0 |
|a YAC Protocols
|c by David Markie.
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| 250 |
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|a 1st ed. 1996.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1996.
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| 300 |
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|a XVIII, 372 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 54
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| 505 |
0 |
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|a Generation of Large Insert YAC Libraries -- YAC Library Storage and Transport -- YAC Library Screening I -- YAC Library Screening II -- Cloning of Human Telomeres in Saccharomyces cerevisiae -- Purification of YAC-Containing Total Yeast DNA -- Restriction Analysis of YACs -- RARE-Cleavage Analysis of YACs -- YAC Localization by Fluorescence In Situ Hybridization -- Alu-PCR Fingerprinting of YACs -- Detection of Chimerism in YAC Clones -- Amplification with Arbitrary Primers -- End Rescue from YACs Using the Vectorette -- Isolation of YAC Ends by Plasmid Rescue -- End-Rescue of YAC Clone Inserts by Inverse PCR -- Covering YAC-Cloned DNA with Phages and Cosmids -- Fragmentation and Integrative Modification of YACs -- Targeting Mutations to YACs by Homologous Recombination -- Reconstruction of Large Genomic Segments of DNA by Meiotic Recombination Between YACs -- Genomic Reconstruction by Mitotic Recombination of YACs -- Amplification of the Copy Number of YACs -- Transfer of YAC Clones to New Yeast Hosts -- Use of ACEDB as a Database for YAC Library Data Management -- YAC Transfer into Mammalian Cells by Cell Fusion -- YAC Transfer by Microinjection -- Transfection of Mammalian Cells via Lipofection -- The Isolation of cDNAs by Hybridization of YACs to cDNA Libraries -- cDNA Selection with YACs -- Markers, Selection, and Media in YAC Cloning.
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| 650 |
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|a Human genetics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
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|u https://doi.org/10.1385/0896033139
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