Protein Purification Protocols - Doonan, Shawn; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1996)
General Strategies -- Preparation of Extracts from Animal Tissues -- Protein Extraction from Plant Tissues -- Extraction of Recombinant Protein from Bacteria -- Protein Extraction from Fungi -- Subcellular Fractionation of Animal Tissues -- Subcellular Fractionation of Plant Tissues -- The Extraction of Enzymes from Plant Tissues Rich in Phenolic Compounds -- Avoidance of Proteolysis in Extracts -- Concentration of Extracts -- Making and Changing Buffers -- Purification and Concentration by Ultrafiltration -- Bulk Purification by Fractional Precipitation -- Ion-Exchange Chromatography -- Hydrophobic Interaction Chromatography -- Affinity Chromatography -- Dye-Ligand Affinity Chromatography -- Lectin Affinity Chromatography -- Immunoaffinity Chromatography -- Immobilized Metal Ion Affinity Chromatography -- Chromatography on Hydroxyapatite -- Affinity Precipitation Methods -- Isoelectric Focusing -- Chromatofocusing -- Size-Exclusion Chromatography -- Fast Protein Liquid Chromatography (FPLC) Methods -- Reversed-Phase Chromatography of Proteins -- Extraction of Membrane Proteins -- Removal of Detergent from Protein Fractions -- Purification of Membrane Proteins -- Lyophilization of Proteins -- Storage of Pure Proteins -- Electroelution of Proteins from Polyacrylamide Gels -- Electroblotting of Proteins from Polyacrylamide Gels -- High-Performance Electrophoresis Chromatography -- Practical Column Chromatography.
Keywords: Cell biology; Cell Biology