Recombinant Gene Expression Protocols - Tuan, Rocky S.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1997)
Recombinant Gene Expression Protocols -- Expression in E. Coli -- Designing Expression Plasmid Vectors in E. coli -- Design of Bacterial Hosts for lac-Based Expression Vectors -- Bacteriophage ?-Based Expression Vectors -- Strategies for Gene Fusions -- Application of the E. coli trp Promoter -- Expression of Proteins in E. coli Utilizing a Dual Promoter-Based Vector: pLACT7 -- Expression and Secretion of Proteins in E. coli -- Synthetic Two-Cistron Expression System -- Expression of cDNA-Encoded Proteins by Cell-Free Transcription and Translation -- Expression in Yeast -- Yeast Plasmids -- Inducible Expression Cassettes in Yeast: GAL4 -- Inducible Expression Cassettes in Yeast: ADH2 -- Constitutive Expression Vectors: PGK -- Viral Expression Systems -- Design and Construction of Recombinant Vaccinia Viruses -- A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus -- Design of Retroviral Expression Vectors -- Use of Defective Herpes-Derived Plasmid Vectors -- Expression in Nonmammalian Higher Eukaryotes -- Use of Baculovirus Expression Vectors -- Expression of Exogenous Genes in Xenopus Oocytes, Eggs, and Embryos -- Analysis of Heterologous Gene Expression in Xenopus Blastomeres -- Expression in Mammalian Cells -- Overview of Vector Design for Mammalian Gene Expression -- Experimental Strategies in Efficient Transfection of Mammalian Cells -- Experimental Strategies in Efficient Transfection of Mammalian Cells -- Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer -- Selection of Transfected Cells -- Selection of Transfected Cells and Coamplification of Transfected Genes -- Optimization of Growth, Viability, and Specific Productivity for Expression of Recombinant Proteins in Mammalian Cells -- Expression in Transgenic Organisms -- Promoters to Express Cloned Genes Uniformly in Drosophila -- Application of Micromechanical Piercing Structures for Genetic Transformation of Nematodes -- Strategies in Generating Transgenic Mammals -- Use of Gonadal Primordial Germ Cells (PGCs) as Tools for Gene Transfer in Chickens -- Strategies for the Production of Transgenic Chickens -- Expression in Plants -- Plant Cell Transfection by Electroporation -- Transformation and Foreign Gene Expression in Plants Mediated by Microprojectile Bombardment -- Transformation of the Cereals Using Agrobacterium -- Transformation of Microalgae Using Silicon Carbide Whiskers.
Keywords: Cell biology; Cell Biology