Recombinant Protein Protocols Detection and Isolation - Tuan, Rocky S.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1997)
Overview of Experimental Strategies on the Detection and Isolation of Recombinant Proteins and Their Applications -- Detection and Isolation of Expressed Proteins -- Reporter Systems -- Detection of Recombinant Protein Based on Reporter Enzyme Activity: Chloramphenicol Acetyltransferase -- Human Placental Alkaline Phosphatase as a Marker for Gene Expression -- Use of Secreted Alkaline Phosphatase as a Reporter of Gene Expression in Mammalian Cells -- Detection of ?-Galactosidase and ?-Glucuronidase Using Chemiluminescent Reporter Gene Assays -- Chemiluminescent Immunoassay for the Detection of Chloramphenicol Acetyltransferase and Human Growth Hormone Reporter Proteins -- Detection and Selection of Cultured Cells Secreting Recombinant Product by Soft Agar Cloning and Antibody Overlay -- Detection and Isolation of Recombinant Protein Based on Binding Affinity Reporter -- Detection and Isolation of Recombinant Proteins Based on Binding Affinity of Reporter: Protein A -- Expression and Purification of Recombinant Streptavidin-Containing Chimeric Proteins -- Bacterial Expression, Purification, and Potential Use of His-Tagged GAL4 Fusion Proteins -- Detection of Expressed Recombinant Protein Based on Multidrug Resistance: P-Glycoprotein -- Detection and Isolation of Recombinant Proteins from Mammalian Cells by Immunoaffinity Chromatography: p53 -- Yeast GAL4 Two-Hybrid System -- Alternative Yeast Two-Hybrid Systems -- Detection of Heterologous Gs-Coupled Receptor Activity in LLC-PK1 Cells Based on Expression of Urokinase-Type Plasminogen Activator -- Detection of Recombinant Gene Expression In Situ -- Histochemical and Fluorochrome-Based Detection of ?-Galactosidase -- Combined, Sequential In Situ Hybridization and Immunohistochemistry on the Same Tissue Section -- Whole Mount In Situ Hybridization to Embryos and Embryonic Tissues -- Whole-Mount In Situ Hybridization for Developing Chick Embryos Using Digoxygenin-Labeled RNA Probes -- Methods for Double Detection of Gene Expression -- In Situ PCR -- Expression and Detection of Green Fluorescent Protein (GFP) -- Applications of Recombinant Gene Expression -- Transgenic Animals as Bioreactors for Expression of Recombinant Proteins -- Artificial Cells for Bioencapsulation of Cells and Genetically Engineered E. coli -- Hyperexpression of a Synthetic Protein-Based Polymer Gene -- Transplantation of Cells in an immunoisolation Device for Gene Therapy -- Delivery of Recombinant HIV-1-Directed Antisense and Ribozyme Genes -- Inducible System Designed for Future Gene Therapy -- Human Gene Therapy.
Keywords: Cell biology; Cell Biology