Protein Sequencing Protocols - Smith, Bryan John.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1997)
Strategies for Handling Polypeptides on a Microscale -- SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing -- Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization -- Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization -- Enzymatic Cleavage of Polypeptides -- Chemical Cleavage of Polypeptides -- Preparation of Glycopeptides -- Selective Isolation of the Carboxy Terminal Peptide from a Protein -- Selective Isolation of the Amino-Terminal Peptide from ?-Amino Blocked Protein -- Analytical and Micropreparative Capillary Electrophoresis of Peptides -- Reverse-Phase HPLC Purification of Peptides from Natural Sources for Structural Analysis -- High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues -- Hydrolysis of Samples for Amino Acid Analysis -- Amino Acid Analysis -- Postcolumn Amino Acid Analysis -- Fast-Atom Bombardment and Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins -- Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- N-Terminal Protein Ladder Sequencing -- The Dansyl-Edman Method for Manual Peptide Sequencing -- The Dansyl Method for Identifying N-Terminal Amino Acids -- Identification of PTH-Amino Acids by High-Performance Liquid Chromatography -- Protein Sequencer Maintenance and Troubleshooting -- Identifying the Carboxy Terminal Amino Acid of a Protein -- Enzymatic and Chemical Methods for Manual C-Terminal Peptide Sequencing -- Automated Methods for C-Terminal Protein Sequencing -- Chemical Modification of Proteins for Sequence Analysis -- On-Membrane Deblocking of Proteins -- Analysis of Sites of Protein Phosphorylation -- Identification of Sites of Glycosylation -- Quantification and Location of Disulfide Bonds in Proteins.
Keywords: Analytical chemistry; Analytical Chemistry