PCR Sequencing Protocols - Rapley, Ralph; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1996)
Preparation and Analysis of DNA Sequencing Gels -- Purification of PCR Products from Agarose Gels for Direct Sequencing -- Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing -- Direct Sequencing of Double-Stranded PCR Products with the Sequenase Kit and [?-35 S] dATP -- Direct Sequencing by Thermal Asymmetric PCR -- Rapid Sequencing of cDNA Clones Direct Sequencing Using Sequential Linear/Asymmetric PCR -- Direct Sequencing of PCR Products Using Chemiluminescent Detection -- Direct DNA Sequencing of PCR Products Using Magnetic Beads -- Affinity Capture and Solid-Phase Sequencing of Biotinylated PCR Products -- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing -- Nonradioactive PCR Sequencing Using Digoxigenin -- Silver Sequencing™ -- Direct Sequencing of PCR Products with DNA-Binding Proteins -- PCR Sequencing with the Aid of Detergents -- Direct Sequencing with Highly Degenerate and lnosine-Containing Primers -- Determination of Unknown Genomic Sequences Without Cloning -- DNA Sequencing by the Chemical Method -- Direct PCR Sequencing with Denaturants (Formamide) -- Efficient PCR Production of Single-Stranded DNA Sequencing Templates -- Preparation and Direct Automated Cycle Sequencing of PCR Products -- Solid-Phase Automated Sequencing of PCR-Amplified Genomic DNA -- Cloning PCR Products for Sequencing in M13 Vectors -- Sequencing PCR Products Cloned into M13 Vectors -- Genomic Amplification with Transcript Sequencing (GAWTS) -- DNA Rescue by the Vectorette Method -- Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations.
Keywords: Cell biology; Cell Biology