Epitope Mapping Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Morris, Glenn E. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1996.
Edition:1st ed. 1996.
Series:Methods in Molecular Biology, 66
Subjects:
Online Access:https://doi.org/10.1385/0896033759
Table of Contents:
  • Overview
  • Crystallographic Studies of Antigen-Antibody Interactions
  • Epitope Mapping Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy
  • Mapping Epitopes on Antigens by Immunodiffusion in Gel
  • A Simple Solid-Phase Competition Assay with Labeled Antigen
  • Epitope Mapping by Antibody Competition
  • Epitope Mapping by Surface Plasmon Resonance in the BIAcore
  • Identifying Residues in Antigenic Determinants by Chemical Modification
  • Epitope Mapping by Differential Chemical Modification of Antigens
  • Epitope Mapping by Proteolysis of Antigen-Antibody Complexes
  • Proteolytic Fragmentation for Epitope Mapping
  • Epitope Mapping by Chemical Fragmentation
  • Probing Antibody-Antigen Interactions by Mass Spectrometry
  • Epitope Mapping Using Multipin Peptide Synthesis
  • SPOT Synthesis
  • Tea Bag Synthesis of Positional Scanning Synthetic Combinatorial Libraries and Their Use for Mapping Antigenic Determinants
  • Epitope Mapping Using Phage-Displayed Peptide Libraries
  • Reiterative Screening of Phage-Display Peptide Libraries with Antibodies
  • Homolog Scanning
  • Epitope Mapping Using an Oligonucleotide Replacement Method
  • Epitope Mapping by Region-Specified PCR Mutagenesis
  • Random Fragment Libraries Using Yeast Expression Plasmid
  • Epitope Mapping Using Random Fragment Expression Libraries in ? Phages
  • Random Fragment Libraries Displayed on Filamentous Phage
  • Epitope Mapping by Expression of Restriction Enzyme or PCR Fragments in Bacterial Plasmids
  • Epitope Mapping of Protein Antigens by Expression-PCR (E-PCR)
  • Epitope Mapping on Extracellular Domains of Cell-Surface Proteins Using Exonuclease III
  • An Improved Method for Mapping Epitopes of Recombinant Antigens by Transposon Mutagenesis
  • Incomplete Polypeptides of In Vitro Translation for Epitope Localization
  • T-Cell Epitope Mapping with Synthetic Peptides and Peripheral Blood Mononuclear Cells
  • Use of Natural or Selected Mutants and Variants for Epitope Mapping
  • Production of Panels of Monoclonal Antibodies by the Hybridoma Method
  • Production of Phage-Display Antibodies for Epitope Mapping.