cDNA Library Protocols - Cowell, Ian G.; Austin, Caroline A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1997)
cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR -- Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations -- Isolation of Messenger RNA from Plant Tissues -- cDNA Library Construction for the Lambda ZAP®-Based Vectors -- Clone Excision Methods for the Lambda ZAP®-Based Vectors -- Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs -- Inverse PCR Approach to Cloning cDNA Ends -- Cloning Gene Family Members Using the Polymerase Chain Reaction with Degenerate Oligonucleotide Primers -- Subtractive Hybridization for the Isolation of Differentially Expressed Genes Using Magnetic Beads -- Preparation of Competent Cells for High-Efficiency Plasmid Transformation of Escherichia coli -- The Tetramethylammonium Chloride (TMAC) Method for Screening cDNA Libraries with Highly Degenerate Oligonucleotide Probes Obtained by Reverse Translation of Amino Acid Sequences -- Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides -- Immunological Screening of ?-Phage cDNA Expression Libraries -- Cloning Sequence-Specific DNA-Binding Factors from cDNA Expression Libraries Using Oligonucleotide Binding Site Probes -- Protein Interaction Cloning by Far-Western Screening of ?-Phage cDNA Expression Libraries -- Yeast Two-Hybrid Library Screening -- Signal Sequence Trap -- Isolation of Genetic Suppressor Elements (GSEs) from Random Fragment cDNA Libraries in Retroviral Vectors -- Expression and Preparation of Fusion Proteins from Recombinant ?gt1.1 Phages -- Computer Analysis of Cloned Sequences -- The TIGR Human cDNA Database -- Searching the dbEST Database -- Reference cDNA Library Facilities Available from European Sources.
Keywords: Cell biology; Immunology; Cell Biology; Immunology