Neuropeptide Protocols - Irvine, G. Brent; Williams, Carvell H.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1997)
Preparation of Neuropeptide-Containing Fractions from Biological Materials -- Purification of Extracted Peptides for Structural Analysis -- Amino Acid Sequencing of Neuropeptides -- Neuropeptide Gene Identification Using the Polymerase Chain Reaction -- Solid-Phase Synthesis of Neuropeptides by Fmoc Strategies -- Incorporation of Stable Pseudopeptide Bonds -- Synthesis of Conformationally Restricted Peptides -- Purification of Synthetic Peptides by High Performance Liquid Chromatography -- Molecular Weight Estimation for Neuropeptides Using Size-Exclusion High Performance Liquid Chromatography -- Molecular Weight Determinations Using Polyacrylamide Gel Electrophoresis with Tris-Tricine Buffers -- Determination of Neuropeptides by Capillary Electrophoresis -- Characterization of Neuropeptide Processing by Fast Atom Bombardment Mass Spectrometry -- Analysis of Neuropeptides by Size-Exclusion HPLC Linked to Electrospray Ionization Mass Spectrometry -- Identification of Peptides by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) and Direct Analysis of the Laterobuccal Nerve from the Pond Snail Lymnaea stagnalis -- Use of Circular Dichroism to Determine Secondary Structure of Neuropeptides -- 1H Nuclear Magnetic Resonance (NMR) in the Elucidation of Peptide Structure -- The Study of Membrane- or Receptor-Bound Neuropeptides by NMR -- Molecular Modeling of Neuropeptides -- Tritium Labeling of Neuropeptides -- The Use of IODO-GEN for Preparing 125I-Labeled Peptides and Their Purification by Reversed-Phase High Performance Liquid Chromatography -- Production of Antisera Using Peptide Conjugates -- Radioimmunoassay -- Enzyme-Linked Immunosorbent Assay of Peptides -- Sample Preparation for Peptide Immunocytochemistry -- lmmunocytochemical Methods for Regulatory Peptides -- Ultrastructural Localization of Peptides Using Immunogold Labeling -- Preparation of a Membrane Fraction for Receptor Studies and Solubilization of Receptor Proteins with Retention of Biological Activity -- Radioligand Binding Using 125Labeled Peptides -- Analysis of Data from "Cold Saturation" Radioligand Binding Experiments -- Organ/Tissue Preparations for the Assessment of Agonist/Antagonist Activity -- Measurement of Efflux Rates from Brain to Blood -- Assay of Neuropeptidases Using Fluorogenic Substrates -- Characterization of Neuropeptidases Using Inhibitors.
Keywords: Neurosciences; Neurosciences