Protein Synthesis Methods and Protocols - Martin, Robin; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1998)
Analyses of Ribosome Distribution During In Vitro Translation -- Allele-Specific Priming in the Mapping of rRNA -- Permeabilized Mammalian Cells as a System for Protein Synthesis -- The Measurement of Processivity Errors in Protein Synthesis -- A Biosynthetic Approach for the Incorporation of Unnatural Amino Acids into Proteins -- The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A -- In Vitro Engineering Using Acyl-Derivatized tRNAs -- In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anticodons -- Analysis of rRNA Function Using Specialized Ribosomes -- Determination of the Peptide Elongation Rate In Vivo -- The Measurement of Missense Errors During In Vivo Protein Synthesis -- Heelprinting Analysis of In Vivo Ribosome Pause Sites -- Analysis of elF-2? Kinases in Yeast -- Continuous-Flow Cell-Free Translation, Transcription-Translation, and Replication-Translation Systems -- Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis -- A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors -- Measurement of Ribosomal Accuracy and Proofreading in E. coli Burst Systems -- Measurement of Rate of Protein Synthesis In Vitro -- In Vivo Mutational Analysis of Ribosomal RNA in Saccharomyces cerevisiae -- Genetic Selection of rRNA Mutations -- Toeprinting Assays -- Mapping of Pseudouridine Residues in RNA to Nucleotide Resolution -- Chemical and Enzymatic Probing of Antibiotic-Ribosome Complexes -- Protein Engineering with Nonstandard Amino Acids -- Internal Ribosome Entry Sites Tests with Circular mRNAs -- Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5? or 3? UTRs of Translationally Regulated mRNAs -- Translational Control by Repressor Proteins Binding to the 5?UTR of mRNAs -- Identification and Analysis of Frameshift Sites -- Synthesis and Site-Specific Binding of Thioated tRNAs to Probe Ribosome-tRNA Interactions.
Keywords: Biochemistry; Biochemistry, general