Plant Virology Protocols From Virus Isolation to Transgenic Resistance - Foster, Gary D.; Taylor, Sally; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1998)
to Plant Virology -- to Classical Crossprotection -- History of Coat Protein-Mediated Protection -- Virus Isolation, RNA Extraction, and Location of Coat Protein Genes -- Geminivirus Isolation and DNA Extraction -- Caulimovirus Isolation and DNA Extraction -- Reovirus Isolation and RNA Extraction -- Procedures for Plant Rhabdovirus Purification, Polyribosome Isolation, and Replicase Extraction -- Hordeivirus Isolation and RNA Extraction -- Furovirus Isolation and RNA Extraction -- Tobravirus Isolation and RNA Extraction -- Tobamovirus Isolation and RNA Extraction -- Potexvirus Isolation and RNA Extraction -- Carlavirus Isolation and RNA Extraction -- Potyvirus Isolation and RNA Extraction -- Trichovirus Isolation and RNA Extraction -- Ilarvirus Isolation and RNA Extraction -- Bromovirus Isolation and RNA Extraction -- Cucumovirus Isolation and RNA Extraction -- Nepovirus Isolation and RNA Extraction -- Comovirus Isolation and RNA Extraction -- Carmovirus Isolation and RNA Extraction -- Tymovirus Isolation and Genomic RNA Extraction -- Tombusvirus Isolation and RNA Extraction -- Luteovirus Isolation and RNA Extraction -- Cloning of Coat Protein Genes -- RNA Analysis -- RNA Fractionation by Density Gradient Centrifugation -- cDNA Library Construction for the Lambda ZAP®-Based Vectors -- PCR Cloning of Coat Protein Genes -- Antibody Production -- Expression Library Screening -- In Vitro Transcription and Translation -- Analysis of Coat Protein Expression Cassettes in Protoplasts -- DNA Sequencing -- Computer Analysis of Amino Acid Sequences -- Plant Transformation and Primary Analysis -- Preparation of Coat Protein-Containing Binary Vectors for Use in Agrobacterium-Mediated Transformation -- Potato Transformation -- Transformation of Tomato -- Tobacco Transformation -- Genetic Transformation of Wheat -- Production of Transgenic Rice (Oryza sativa subspecies japonica cv. Taipei 309) -- Molecular Analysis of Transgenic Rice -- PCR Analysis of Transgenic Tobacco Plants -- Southern Analysis of Transgenic Tobacco Plants -- Detection and Quantification of Transcript RNA in Transgenic Plants Using Digoxigenin-Labeled cDNA Probes -- Western Analysis of Transgenic Plants -- Evaluation of Resistance -- Assaying Levels of Plant Virus by ELISA -- Detection of Plant RNA Viruses by Nonisotopic Dot-Blot Hybridization -- Detection and Quantification of Plant Viruses by PCR -- Assaying Levels of Virus with Local Lesion Hosts -- Field Testing Resistance of Transgenic Plants -- Agronomic Performance of Transgenic Plants -- Mechanisms of Resistance -- Mechanisms of Resistance -- Mechanisms of RNA-Mediated Resistance to Plant Viruses -- Detection of Risks Associated with Coat Protein Transgenics -- Potential Benefits of the Transgenic Control of Plant Viruses in the United Kingdom.
Keywords: Plant science; Botany; Plant Sciences