Retinoid Protocols - Redfern, Christopher; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1998)
Handling and Analysis of Retinoids -- Properties of Retinoids -- Quantitative Analyses of Naturally Occurring Retinoids -- Detection and Measurement of Retinoic Acid Production by Isolated Tissues Using Retinoic Acid-Sensitive Reporter Cell Lines -- Retinoid-Binding Proteins -- Immunohistochemistry for CRBPs and CRABPs -- Whole-Mount In Situ Hybridization of Mouse Embryos Exposed to Teratogenic Levels of Retinoic Acid -- Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for Cellular Retinoid-Binding Proteins -- Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein -- Expression and Purification of CRABPs from E. coli -- Purification and Fluorescent Titration of Cellular Retinol-Binding Protein -- Fluorometric Titration of the CRABPs -- Expression and Mutagenesis of Retinol-Binding Protein -- Interactions of Retinol-Binding Protein with Transthyretin and Its Receptor -- Detection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited Proteolysis -- Measurement of Rates of Dissociation of Retinoids from the Interphotoreceptor Retinoid-Binding Protein -- Use of Antisense Oligonucleotides to Study the Role of CRABPs in Retinoic Acid-Induced Gene Expression -- Nuclear Retinoid Receptors -- Preparation of Polyclonal Antibodies to Retinoid Receptors -- Detection of RARs and RXRs in Cells and Tissues Using Specific Ligand-Binding Assays and Ligand-Binding lmmunoprecipitation Techniques -- Nonisotopic In Situ Hybridization for the Detection of Nuclear Retinoid Receptor Transcripts in Tissue Sections -- In Situ Hybridization with 35S-Labeled Probes for Retinoid Receptors -- Isolation of Retinoid Receptors from Manimalian Cells -- Analysis of Retinoid Receptor Phosphorylation -- Photoaffinity Labeling of RARs and Mapping of Labeled Sites by an Endoproteinase Combination Technique -- PCR Cloning of N-Terminal RAR Isoforms and APL-Associated PLZF-RAR? Fusion Proteins -- RT-PCR in Diagnosis and Disease Monitoring of Acute Promyelocytic Leukemia (APL) -- A Two-Hybrid Protein Interaction System to Identify Factors That Interact with Retinoid and Vitamin D Receptors -- Gel-Shift Analysis and Identification of RXREs and RAREs by PCR-Based Selection -- Identification and Cloning of RA-Regulated Genes by mRNA-Differential Display -- Gene Targeting of Retinoid Receptors.
Keywords: Cell biology; Cell Biology