Bioluminescence Methods and Protocols - LaRossa, Robert A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1998)
The Basics -- Improvements in the Application of Firefly Luciferase Assays -- Visualization of Bioluminescence -- Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gone -- Analytical Biochemistry -- Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue -- Bioluminescent Assay of the Guanylates -- Bioluminescent Assay of the Adenylate Energy Charge -- Molecular Biology Tools -- Photorhabdus luminescens luxCDABE Promoter Probe Vectors -- Insertion of Promoter Region::luxCDABE Fusions into the Escherichia coil Chromosome -- Probing for Promoters with Luciferase-Transposons -- Cryopreservation and Reawakening -- Luciferase Renaturation Assays of Chaperones and Chaperone Antagonists -- Cell-Based Assays -- Genotoxic Sensors -- Stress Detection Using Bioluminescent Reporters of the Heat-Shock Response -- Real-Time Reporter of Protein Synthesis Inhibition -- Luminescence-Based Cell Viability Testing -- Phagocyte Chemiluminescence -- Detection of Oxidants Using lux Fusions to Oxidative Stress Promoters -- Environmental Applications -- Luciferase-Based Measurement of Water Contaminants -- Bioluminescence-Based Metal Detectors -- Luminescence Facilitated Detection of Bioavailable Mercury in Natural Waters -- A Panel of Stress-Responsive Luminous Bacteria for Monitoring Wastewater Toxicity -- Organic Contaminant Detection and Biodegradation Characteristics -- Detection of Firefly Luciferase-Tagged Bacteria in Environmental Samples -- Monitoring of GFP-Tagged Bacterial Cells.
Keywords: Biochemistry; Biochemistry, general