Cytochrome P450 Protocols - Phillips, Ian R.; Shephard, Elizabeth A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1998)
The Role of Methods in the Discovery of the Cytochromes P450 -- Cytochrome P450 Nomenclature -- Spectral Analyses of Cytochromes P450 -- Purification of Cytochromes P450 -- Isolation and Purification of Constitutive Forms of Microsomal Cytochrome P450 -- Purification of Extrahepatic Cytochromes P450 -- Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems -- Cytochrome P450 Reconstitution Systems -- Catalytic Assays for Human Cytochrome P450 -- Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 with 7-Ethoxyresorufin as Substrate -- Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation -- Determination of the CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes -- High-Performance Liquid Chromatographic Analysis of CYP2CSCatalyzed Paclitaxel 6?-Hydroxylation -- Determination of CYP2C9-Catalyzed Diclofenac 4?-Hydroxylation by High-Performance Liquid Chromatography -- CYP2Cl g-Mediated (S)-Mephenytoin 4'-Hydroxylation Assayed by High-Performance Liquid Chromatography with Radiometric Detection -- CYP2D6-Dependent Bufuralol 1'-Hydroxylation Assayed by Reversed-Phase lon-Pair High-Performance Liquid Chromatography with Fluorescence Detection -- Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation -- Thin-Layer Chromatographic Analysis of Human CYP3A-Catalyzed Testosterone 6?-Hydroxylation -- Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography with Radiometric Detection -- An Isocratic High-Performance Liquid Chromatographic Assay for CYP7Al-Catalyzed Cholesterol 7?-Hyciroxylation -- Use of 7-Ethoxycoumarinto Monitor Multiple Enzymesin the Human CYP1, CYP2, and CYP3 Families -- Expression of Eukaryotic Cytochromes P450 in E. coli -- Expression of Mammalian Cytochromes P450 in Yeast -- Expression of Cytochromes P450 in a Baculovirus System -- Expression of Cytochromes P450 in Mammalian Cells -- Monoclonal Antibodies to Cytochromes P450 -- Targeting Antipeptide Antibodies Towards Cytochrome P450 Enzymes -- Signals for Retention of Cytochrome P450 in the Endoplasmic Reticulum -- Quantification of Cytochrome P450 Gene Expression by RNase Protection Analysis -- Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing -- Isolation of Rat Hepatocytes -- Collagen-Gel Cultures of Rat Hepatocytes: -- Rat Hepatocyte Cultures -- Isolation of Periportal and Pericentral Hepatocytes -- Perifusion Culture of Hepatocytes -- Human Hepatocyte Culture -- Reconstruction of Human Epidermis In Vitro -- Gene Transfer and Expression -- Lipid-Mediated Gene Transfer into Normal Adult Human Hepatocytes in Primary Culture -- Identification of Regulatory Protein- Binding Sites in Cytochrome P450 Genes by Means of the Gel- Retardation Assay -- Cytochrome P450 Gene Regulation -- Analysis of Cytochrome P450 Polymorphisms -- Induction of Cytochrome P4501A (CYP1A) in Fish A Biomarker for Environmental Pollution -- Generation of Allelic Series in Mice by Gene Targeting.
Keywords: Biochemistry; Biochemistry, general