Lipase and Phospholipase Protocols - Doolittle, Mark.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1999)
Lipase Activity, Purification, and Expression -- Phospholipase A2 and Phosphatidylinositol-Specific Phospholipase C Assays by HPLC and TLC with Fluorescent Substrate -- Fluorometric Phospholipase Assays Based on Polymerized Liposome Substrates -- Triglyceride Lipase Assays Based on a Novel Fluorogenic Alkyldiacyl Glycerol Substrate -- Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2 -- Determination of Plasmalogen-Selective Phospholipase A2 Activity by Radiochemical and Fluorometric Assay Procedures -- Human Plasma Platelet-Activating Factor Acetylhydrolase -- Assays for Pancreatic Triglyceride Lipase and Colipase -- Bile Salt-Activated Lipase -- Determining Lipoprotein Lipase and Hepatic Lipase Activity Using Radiolabeled Substrates -- Lysosomal Acid Lipase -- Hormone-Sensitive Lipase and Neutral Cholesteryl Ester Lipase -- Lecithin-Cholesterol Acyltransferase -- Purification of Lipases and Phospholipases by Heparin-Sepharose Chromatography -- Large-Scale Lipoprotein Lipase Purification from Adipose Tissue -- Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B -- High-Level Expression and Purification of Human Hepatic Lipase from Mammalian Cells -- High-Level Baculoviral Expression of Hormone-Sensitive Lipase -- High-Level Baculoviral Expression of Lysosomal Acid Lipase -- One-Step Purification and Biochemical Characterization of Recombinant Pancreatic Lipases Expressed in Insect Cells -- Modulation of the Expression Level of Human Acidic Lipases by Various Signal Peptides -- Biochemical Characterization -- Immunodetection of Lipoprotein Lipase: Antibody Production, Immunoprecipitation, and Western Blotting Techniques -- Immunological Characterization of Digestive Lipases -- Determining Lipase Subunit Structure by Sucrose Gradient Centrifugation -- Detecting Ligands Interacting with Lipoprotein Lipase -- Monolayer Techniques for Studying Lipase Kinetics -- Efficient Immobilization of Phospholipase A2 -- Molecular Genetic Characterization -- An Introduction to Internet Resources for the Molecular and Genetic Analysis of the Lipases -- Techniques for the Measurement of Lipoprotein Lipase Messenger RNA -- In Vitro Transcription and Translation of Lipoprotein Lipase -- Induced Lipase Mutations in the Mouse.
Keywords: Biochemistry; Biochemistry, general