Herpes Simplex Virus Protocols - Brown, S. Moira; MacLean, Alasdair R.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1998)
HSV Growth, Preparation, and Assay -- HSV Entry and Spread -- Preparation of HSV-DNA and Production of Infectious Virus -- HSV Mutagenesis -- Saturating Mutagenesis and Characterization of a Herpesvirus Genome Using In Vivo Reconstitution of Virus from Cloned Subgenomic Regions -- Construction and Use of Cell Lines Expressing HSV Genes -- Analysis of HSV Polypeptides -- Protein Purification -- Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells -- Crystallization of Macromolecules for Three-Dimensional Structure Determination -- Direct Immunogold Labeling of Herpesvirus Suspensions -- Expression of HSV Proteins in Bacteria -- In Vitro Systems to Analyze HSV Transcript Processing -- Analysis of HSV-1 Transcripts by RNA-PCR -- Transient Assays for HSV Origin and Replication Protein Function -- HSV Amplicons in Gene Therapy -- Analyses of HSV Proteins for Posttranslational Modifications and Enzyme Functions -- HSV-Cellular Protein Interactions -- Models of Recurrent Infection with HSV in the Skin and Eye of the Mouse -- Pathogenesis and Molecular Biology of HSV Latency and Ocular Reactivation in the Rabbit -- HSV Latency In Vitro -- Assessing Cell-Mediated Immune Responses to HSV in Murine Systems -- Assays for HSV Gene Expression During Establishment and Maintenance of Latent Infection -- Analysis of HSV-DNA and RNA Using the Polymerase Chain Reaction -- HSV Vectors for Gene Therapy -- Investigation of the Anti-HSV Activity of Candidate Antiviral Agents.
Keywords: Microbiology; Microbiology