HIV Protocols - Michael, Nelson; Kim, Jerome H.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1999)
Virology -- Isolation and Expansion of HIV from Cells and Body Fluids by Coculture -- Quantitative HIV Culture -- Quantitation of Cell-Free HIV by Reverse Transcriptase Activity -- Determination of Syncytium-Inducing Phenotype of Primary HIV-1 Isolates Using MT-2 cells -- Determination of HIV-1 Chemokine Coreceptor Tropism Using Transduced Human Osteosarcoma (HOS) Cells -- Use of Luciferase Reporter Viruses for Studying HIV Entry -- A Cell-Cell Fusion Assay to Monitor HIV-1 Env Interactions with Chemokine Receptors -- Methods of Culturing HIV-1 from Semen -- Molecular Biology -- Detection of HIV-1 Nucleic Acids by Southern Blotting -- Detection of HIV-1 Nucleic Acids by Northern Blotting -- Rapid Identification of Cloned HIV-1 Fragments -- PCR Amplification and Cloning of Virtually Full-Length HIV-1 Provirus -- Quantitation of HIV-1gag DNA and RNA From Single Frozen Cell Pellets -- Quantitative PCR for HIV-1 Proviral DNA -- Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper -- Quantification of HIV-1 RNA in Dried Plasma Spots (DPS) -- Quantitation of HIV-1 RNA in Genital Secretions -- Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction -- Determination of the Promoter Activity of HIV-1 Using the Chloramphenicol Acetyltransferase Reporter Gene Assay -- In Vitro Techniques for Studies of HIV-1 Promoter Activity -- Detection of Polymorphisms in the HIV-1 Coreceptor CCR5 Using Single-Strand Conformation Polymorphism -- Sequence-Specific Priming as a Rapid Screen for Known Mutations -- Quantitation of HIV-1 Entry Cofactor Expression -- Retrovirus-Mediated Gene Transduction of SupT1 Cells -- Cloning of HIV Single-Strand DNA Binding Protein from Human Lymphocyte Lambda gt11 Expression Library with [32P]-Oligomers -- Telomeric Terminal Restriction Fragment (TRF) -- Humoral Immunology -- Serologic Analysis by Enzyme-Linked Immunosorbent Assay (ELISA) -- Quantitative Immunoblotting with Fusion Proteins -- Analysis of Antibody Interactions with HIV-1 Envelope Expressed on the Surface of Acutely Infected H9 Cells -- Analysis of Antibody-Antigen Interactions Using Surface Plasmon Resonance -- Measurement of HIV-1 Specific and Total Antibody Secreting Cells by ELISPOT -- Linear Epitope Mapping by the PEPSCAN Method -- Neutralization of HIV-1 Infection of Human Peripheral Blood Mononuclear Cells (PBMC) -- Neutralization of HIV-1 Infection of Human Peripheral Blood Mononuclear Cells (PBMC) -- Collection and Processing of Mucosal Secretions from HIV-1 Infected Women -- Collection and Processing of Mucosal Secretions from Mice -- Cellular Immunology -- Lymphocyte Proliferation Assay -- HIV-1-Specific Cytotoxic T-Cell Assays -- HIV-1-Specific Antibody-Dependent Cellular Cytotoxicity (ADCC) -- Evaluation of Natural Killer Cell Activity -- Generation and Expansion of HIV-1 CD4+ Antigen-Specific T Cell Lines -- Immunophenotyping and Assessment of Cell Function by Three-Color Flow Cytometry of Peripheral Blood Lymphocytes -- Detection of Apoptosis in HIV-Infected Ceil Populations using TUNEL.
Keywords: Immunology; Immunology