Molecular Biomethods Handbook - Rapley, Ralph; Walker, John M.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1998)
Extraction of Total RNA from Tissues and Cultured Cells -- Isolation of Total Cellular DNA from Tissues and Cultured Cells -- Gel Electrophoresis of DNA -- Sl Nuclease Analysis of RNA -- Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA) -- Gene Probes -- Nonradioactive Labeling of DNA -- Southern Blot Analysis -- Northern Blot Analysis -- DNA Sequencing -- Autoradiography and Fluorography -- Mobility Shift Assays -- cDNA Libraries -- Genomic DNA Libraries -- ? as a Cloning Vector -- Plasmid-Derived Cloning Vectors -- M13 and Phagemid-Based Cloning Vectors -- Retroviral Vectors From Laboratory Tools to Molecular Medicines -- Baculovirus Vectors -- Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes -- Plant Transformation -- Restriction Fragment-Length Polymorphisms -- Genome Mapping -- Yeast Artificial Chromosomes -- Polymerase Chain Reaction -- In Vitro Transcriptions -- In Vitro Translation -- Site-Directed Mutagenesis -- Site-Directed Mutagenesis -- DNA Profiling -- Radiolabeling of Peptides and Proteins -- Protein Electrophoresis -- Free Zone Capillary Electrophoresis -- Protein Blotting -- Ion-Exchange Chromatography -- Size-Exclusion Chromatography -- Hydrophobic Interaction Chromatography -- Affinity Chromatography -- Reversed-Phase HPLC -- Glycoprotein Analysis -- Protein Sequencing -- Solid-Phase Peptide Synthesis -- Protein Engineering -- Monoclonal Antibodies -- Phage-Display Libraries -- Enzyme- Linked Immunosorbent Assay (ELISA) -- Epitope Mapping -- lmmunocytochemistry -- Flow Cytometry -- Mass Spectrometry -- The Technique of In Situ Hybridization.
Keywords: Cell biology; Cell Biology