DNA Repair Protocols - Henderson, Daryl S.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1999)
Mutant Isolation and Gene Cloning -- Isolation of DNA Structure-Dependent Checkpoint Mutants in S. pombe -- Isolating Mutants of the Nematode Caenorhabditis elegans That Are Hypersensitive to DNA-Damaging Agents -- Isolating DNA Repair Mutants of Drosophila melanogaster -- Generation, Identification, and Characterization of Repair-Defective Mutants of Arabidopsis -- Screening for ?-Ray Hypersensitive Mutants of Arabidopsis -- Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating -- Strategies for Cloning Mammalian DNA Repair Genes -- Novel Complementation Assays for DNA Repair-Deficient Cells -- Recognition and Removal of Inappropriate or Damaged DNA Bases -- The Use of Electrophoretic Mobility Shift Assays to Study DNA Repair -- Mismatch Repair Assay -- Measurement of Activities of Cyclobutane-Pyrimidine-Dimer and (6-4)-Photoproduct Photolyases -- A Dot Blot Immunoassay for UV Photoproducts -- Measurement of UV Radiation-Induced DNA Damage Using Specific Antibodies -- Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay -- Monitoring Removal of Cyclobutane Pyrimidine Dimers in Arabidopsis -- DNA Damage Quantitation by Alkaline Gel Electrophoresis -- The Comet Assay (Single-Cell Gel Test) -- Measuring the Formation and Repair of UV Photoproducts by Ligation-Mediated PCR -- PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair I -- PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair II -- Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage -- Characterization of DNA Strand Cleavage by Enzymes That Act at Abasic Sites in DNA -- Base Excision Repair Assay Using Xenopus laevis Oocyte Extracts -- In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts -- Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts -- In Vitro Excision Repair Assay in Schizosaccharomyces pombe -- Nucleotide Excision Repair Assay in Drosophila melanogaster Using Established Cell Lines -- Nucleotide Excision Repair in Nuclear Extracts from Xenopus Oocytes -- Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA -- Dual-Incision Assays for Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site -- DNA Strand Breakage and Repair -- In Vitro Chemiluminescence Assay to Measure Excision Repair in Cell Extracts -- Physical Monitoring of HO-Induced Homologous Recombination -- Use of P Element Transposons to Study DNA Double-Strand Break Repair in Drosophila melanogaster -- Analyzing Double-Strand Repair Events in Drosophila -- Expression of I-Sce I in Drosophila to Induce DNA Double-Strand Breaks -- Use of I-Sce I to Induce DNA Double-Strand Breaks in Nicotiana -- Chromosomal Double-Strand Breaks Introduced in Mammalian Cells by Expression of I-Sce I Endonuclease -- Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells -- In Vitro Rejoining of Double-Strand Breaks in Genomic DNA -- Extrachromosomal Assay for DNA Double-Strand Break Repair -- Use of Gene Targeting to Study Recombination in Mammalian DNA Repair Mutants -- DNA Damage Tolerance Mechanisms and Regulatory Responses -- Measurement of Low-Frequency DNA Breaks Using Nucleoid Flow Cytometry -- Live Analysis of the Division Cycles in X-Irradiated Drosophila Embryos -- Inhibition of DNA Synthesis by Ionizing Radiation -- Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication -- Assays of Bypass Replication of Genotoxic Lesions in Mammalian Disease and Mutant Cell-Free Extracts -- Detection of Chromatin-Bound PCNA in Cultured Cells Following Exposure to DNA-Damaging Agents -- Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo -- Activation of p53 Protein Function in Response to Cellular Irradiation -- Selective Extraction of Fragmented DNA from Apoptotic Cells for Analysis by Gel Electrophoresis and Identification of Apoptotic Cells by Flow Cytometry -- Detection of DNA Strand Breakage in the Analysis of Apoptosis and Cell Proliferation by Flow and Laser Scanning Cytometry -- Immunoassay for Single-Stranded DNA in Apoptotic Cells.
Keywords: Biochemistry; Cell biology; Biochemistry, general; Cell Biology