mRNA Processing and Metabolism Methods and Protocols - Schoenberg, Daniel R.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
Using Chromatin Immunoprecipitation to Map Cotranscriptional mRNA Processing in Saccharomyces cerevisiae -- Identifying PhosphoCTD-Associating Proteins -- Imaging Alternative Splicing in Living Cells -- Tobramycin Affinity Tag Purification of Spliceosomes -- Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Alternative Splicing -- Methods for the Analysis of Adenosine-to-Inosine Editing in RNA -- Assaying Nuclear Messenger RNA Export in Human Cells -- RNA Unwinding Assay for DExD/H-Box RNA Helicases -- Approaches for Monitoring Nuclear Translation -- Immunopurification and Analysis of Protein and RNA Components of mRNP in Mammalian Cells -- Gene Expression Analysis of Messenger RNP Complexes -- Using the ?N Peptide to Tether Proteins to RNAs -- An Efficient System for Cap- and Poly(A)-Dependent Translation In Vitro -- A Poly(A) Tail-Responsive In Vitro System for Cap- or IRES-Driven Translation From HeLa Cells -- Assessing Messenger RNA Decapping in Cellular Extracts -- Analysis of RNA Exonucleolytic Activities in Cellular Extracts -- Application of Ligation-Mediated Reverse Transcription Polymerase Chain Reaction to the Identification of In Vivo Endonuclease-Generated Messenger RNA Decay Intermediates -- Biochemical Dissection of RNA Silencing in Plants -- RNA Interference of mRNA Processing Factors in Drosophila S2 Cells -- RNA Interference by Short Hairpin RNAs Expressed in Vertebrate Cells.
Keywords: Cell biology; Cell Biology