Receptor Signal Transduction Protocols - Willars, Gary B.; Challiss, R. A. John.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
Radioligand-Binding Methods for Membrane Preparations and Intact Cells -- Determination of Allosteric Interactions Using Radioligand-Binding Techniques -- Generation, Use, and Validation of Receptor-Selective Antibodies -- Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization -- Generation and Use of Epitope-Tagged Receptors -- Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and In Situ Hybridization -- Electron Microscopic Studies of Receptor Localization -- Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors -- Viral Infection Protocols -- Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection -- Covalent Modification of G-Proteins by Affinity Labeling -- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Assess Total G-Protein and G?-Subtype-Specific Activation by G-Protein-Coupled Receptors -- Identification and Quantitation of G-Protein ?-Subunits -- Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins -- Assessment of Receptor Internalization and Recycling -- G-Protein-Coupled Receptor Phosphorylation and Palmitoylation -- Identification of G-Protein-Coupled Receptor Phosphorylation Sites by 2D Phosphopeptide Mapping -- Ubiquitination of G-Protein-Coupled Receptors -- Receptor Mutagenesis Strategies for Examination of Structure-Function Relationships -- Study of G-Protein-Coupled Receptor-Protein Interactions by Bioluminescence Resonance Energy Transfer -- Fluorescence Resonance Energy Transfer to Study Receptor Dimerization in Living Cells -- Identification of Protein Interactions by Yeast Two-Hybrid Screening and Coimmunoprecipitation -- Study of G-Protein-Coupled Receptor-Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Protein Pull-Downs -- Receptor Knock-Out and Knock-In Strategies -- Statistical Methods in G-Protein-Coupled Receptor Research.
Keywords: Cell biology; Cell Biology