Protein'Protein Interactions Methods and Applications /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Fu, Haian. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edición:1st ed. 2004.
Colección:Methods in Molecular Biology, 261
Materias:
Acceso en línea:https://doi.org/10.1385/1592597629
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245 1 0 |a Protein'Protein Interactions  |b Methods and Applications /  |c edited by Haian Fu. 
250 |a 1st ed. 2004. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2004. 
300 |a XVI, 532 p.  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 261 
505 0 |a Overview -- Structural Basis of Protein-Protein Interactions -- Quantitative Analysis of Protein-Protein Interactions -- In Vitro Techniques -- Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry -- Circular Dichroism Analysis for Protein-Protein Interactions -- Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy -- Measuring Rhodopsin-G-Protein Interactions by Surface Plasmon Resonance -- Using Light Scattering to Determine the Stoichiometry of Protein Complexes -- Sedimentation Equilibrium Studies -- Analysis of Protein-Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography -- Fluorescence Gel Retardation Assay to Detect Protein-Protein Interactions -- Fluorescence Polarization Assay to Quantify Protein-Protein Interactions -- Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot -- Glutathione-S-Transferase-Fusion Based Assays for Studying Protein-Protein Interactions -- Affinity Capillary Electrophoresis Analyses of Protein-Protein Interactions in Target-Directed Drug Discovery -- Mapping Protein-Ligand Interactions by Hydroxyl-Radical Protein Footprinting -- Use of Phage Display and Polyvalency to Design Inhibitors of Protein-Protein Interactions -- Detecting Protein-Protein Interactions in Heterologous Systems -- A Bacterial Two-Hybrid System Based on Transcription Activation -- Using the Yeast Two-Hybrid System to Identify Interacting Proteins -- Analysis of Protein-Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System -- The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System -- Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae -- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo -- Co-Immunoprecipitation from Transfected Cells -- Probing Protein-Protein Interactions in Living Cells -- Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET) -- Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer -- Fluorescence Correlation Spectroscopy -- Confocal Microscopy for Intracellular Co-Localization of Proteins -- Mapping Biochemical Networks With Protein-Fragment Complementation Assays -- In Vivo Protein Cross-Linking -- Proteomics-Based Approaches -- Computational Prediction of Protein-Protein Interactions -- Affinity Methods for Phosphorylation-Dependent Interactions -- Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes -- Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry -- Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry -- Internet Resources for Studying Protein-Protein Interactions. 
650 0 |a Biochemistry. 
650 1 4 |a Biochemistry, general. 
700 1 |a Fu, Haian.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1385/1592597629