Protein'Protein Interactions Methods and Applications - Fu, Haian; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
Overview -- Structural Basis of Protein-Protein Interactions -- Quantitative Analysis of Protein-Protein Interactions -- In Vitro Techniques -- Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry -- Circular Dichroism Analysis for Protein-Protein Interactions -- Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy -- Measuring Rhodopsin-G-Protein Interactions by Surface Plasmon Resonance -- Using Light Scattering to Determine the Stoichiometry of Protein Complexes -- Sedimentation Equilibrium Studies -- Analysis of Protein-Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography -- Fluorescence Gel Retardation Assay to Detect Protein-Protein Interactions -- Fluorescence Polarization Assay to Quantify Protein-Protein Interactions -- Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot -- Glutathione-S-Transferase-Fusion Based Assays for Studying Protein-Protein Interactions -- Affinity Capillary Electrophoresis Analyses of Protein-Protein Interactions in Target-Directed Drug Discovery -- Mapping Protein-Ligand Interactions by Hydroxyl-Radical Protein Footprinting -- Use of Phage Display and Polyvalency to Design Inhibitors of Protein-Protein Interactions -- Detecting Protein-Protein Interactions in Heterologous Systems -- A Bacterial Two-Hybrid System Based on Transcription Activation -- Using the Yeast Two-Hybrid System to Identify Interacting Proteins -- Analysis of Protein-Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System -- The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System -- Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae -- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo -- Co-Immunoprecipitation from Transfected Cells -- Probing Protein-Protein Interactions in Living Cells -- Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET) -- Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer -- Fluorescence Correlation Spectroscopy -- Confocal Microscopy for Intracellular Co-Localization of Proteins -- Mapping Biochemical Networks With Protein-Fragment Complementation Assays -- In Vivo Protein Cross-Linking -- Proteomics-Based Approaches -- Computational Prediction of Protein-Protein Interactions -- Affinity Methods for Phosphorylation-Dependent Interactions -- Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes -- Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry -- Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry -- Internet Resources for Studying Protein-Protein Interactions.
Keywords: Biochemistry; Biochemistry, general