Recombinant Gene Expression Reviews and Protocols - Balbas, Paulina; Lorence, Argelia; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
General Issues About Recombinant Gene Expression -- Host Cell Compatibility in Protein Expression -- Production of Recombinant Proteins -- Folding-Promoting Agents in Recombinant Protein Production -- Prokaryotes -- Back to Basics -- ?-Complementation-Enabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experiments -- Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli -- Overexpression of Chromosomal Genes in Escherichia coli -- Chromosomal Expression of Foreign and Native Genes From Regulatable Promoters in Escherichia coli -- Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli -- Copy-Control pBAC/oriV Vectors for Genomic Cloning -- Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV -- Cell-Free Protein Synthesis With Prokaryotic Combined Transcription-Translation -- Genetic Tools for the Manipulation of Moderately Halophilic Bacteria of the Family Halomonadaceae -- Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria -- Recombinant Protein Production in Antarctic Gram-Negative Bacteria -- Fungi -- Recombinant Protein Production in Yeasts -- Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae -- High-Throughput Expression in Microplate Format in Saccharomyces cerevisiae -- High-Throughput Expression in Microplate Format in Pichia pastoris -- Multiple Gene Expression by Chromosomal Integration and CRE-loxP-Mediated Marker Recycling in Saccharomyces cerevisiae -- Three Decades of Fungal Transformation -- Three Decades of Fungal Transformation -- Plants and Plant Cells -- Gene Transfer and Expression in Plants -- Production of Recombinant Proteins by Hairy Roots Cultured in Plastic Sleeve Bioreactors -- Engineering the Chloroplast Genome for Hyperexpression of Human Therapeutic Proteins and Vaccine Antigens -- New Selection Marker for Plant Transformation -- Enhancer Detection and Gene Trapping as Tools for Functional Genomics in Plants -- Animals and Animal Cells -- Gene Transfer and Expression in Mammalian Cell Lines and Transgenic Animals -- Sustained Heterologous Transgene Expression in Mammalian and Avian Cell Lines -- Inducible Gene Expression in Mammalian Cells and Mice -- Flp-Mediated Integration of Expression Cassettes into FRT-Tagged Chromosomal Loci in Mammalian Cells -- Generation of High-Recombinant-Protein-Producing Chinese Hamster Ovary (CHO) Cells -- Preparation of Recombinant Proteins in Milk.
Keywords: Cell biology; Cell Biology