Parasite Genomics Protocols - Melville, Sara E.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
Sequencing Strategies for Parasite Genomes -- Annotation of Parasite Genomes -- Parasite Genome Databases and Web-Based Resources -- Expressed Sequence Tags -- Expressed Sequence Tags -- Positive Selection Scanning of Parasite DNA Sequences -- RACE and RAGE Cloning in Parasitic Microbial Eukaryotes -- Amplified (Restriction) Fragment Length Polymorphism (AFLP) Analysis -- Minisatellites and MVR-PCR for the Individual Identification of Parasite Isolates -- Analysis of Differentially Expressed Parasite Genes and Proteins Using Transcriptomics and Proteomics -- Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays -- DNA Content Analysis on Microarrays -- Typing Single-Nucleotide Polymorphisms in Toxoplasma gondii by Allele-Specific Primer Extension and Microarray Detection -- Transfection of the Human Malaria Parasite Plasmodium falciparum -- A PCR-Based Method for Gene Deletion and Protein Tagging in Trypanosoma brucei -- Analysis of Gene Function in Trypanosoma brucei Using RNA Interference -- In Vitro Shuttle Mutagenesis Using Engineered Mariner Transposons -- Random Mutagenesis Strategies for Construction of Large and Diverse Clone Libraries of Mutated DNA Fragments -- Separation, Digestion, and Cloning of Intact Parasite Chromosomes Embedded in Agarose -- Chromosome Fragmentation in Leishmania -- FISH Mapping for Helminth Genome -- Fiber-FISH: Fluorescence In Situ Hybridization on Stretched DNA -- Yeast Two-Hybrid Assay for Studying Protein-Protein Interactions -- From Genomes to Vaccines for Leishmaniasis.
Keywords: Microbiology; Microbiology