Photosynthesis Research Protocols - Carpentier, Robert; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
Fractionation of Thylakoid Membranes Into Grana and Stroma Thylakoids -- Isolation of Photosystem I Particles From Spinach -- Rapid Isolation and Purification of Photosystem I Chlorophyll-Binding Protein From Chlamydomonas reinhardtii -- Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins From Higher Plants -- Isolation of Functional Photosystem II Core Particles From the Cyanobacterium Synechocystis sp. PCC 6803 -- Isolation of Photosystem I Reaction Center Preparation From Spinach -- Isolation of Photosystem II Reaction Center Complexes From Plants -- Isolation of Photosystem I Reaction Center Subunit Polypeptides From Spinach -- Purification and Crystallization of the Cytochrome b 6 f Complex in Oxygenic Photosynthesis -- Purification of Plastocyanin and Cytochrome c 6 From Plants, Green Algae, and Cyanobacteria -- Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex -- Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies With Light-Inducible Structural Transitions -- Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II -- Isolation of CP43 and CP47 Photosystem II Proximal Antenna Complexes From Plants -- The Determination and Quantification of Photosynthetic Pigments by Reverse Phase High-Performance Liquid Chromatography, Thin-Layer Chromatography, and Spectrophotometry -- Isolation and Identification of Chloroplast Lipids -- DNA Adducts With Chlorophyll and Chlorophyllin As Antimutagenic Agents -- Incorporation and Analysis of LHCII in Model Systems -- Photosystem II Reconstitution Into Proteoliposomes -- Extraction of the Functional Manganese and Calcium From Photosystem II -- Assay of Photoinhibition of Photosystem II and Protease Activity -- Thermoluminescence -- Detection of Free Radicals and Reactive Oxygen Species -- Stabilization of Photosynthetic Materials -- Determination of Phosphoproteins in Higher Plant Thylakoids -- Identifying Photoprotection Mutants in Arabidopsis thaliana -- A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtii -- The Construction of Gene Knockouts in the Cyanobacterium Synechocystis sp. PCC 6803 -- Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation.
Keywords: Plant science; Botany; Plant Sciences