Optimization in Drug Discovery - Yan, Zhengyin; Caldwell, Gary W.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
pKa, Solubility, and Lipophilicity -- Use of Caco-2 Cell Monolayers to Study Drug Absorption and Metabolism -- Absorption Screening Using the PAMPA Approach -- In Situ Single-Pass Perfused Rat Intestinal Model for Absorption and Metabolism -- In Vitro Permeation Study With Bovine Brain Microvessel Endothelial Cells -- An Enzymatic Microplate Assay for Testing P-Glycoprotein Substrates and Inhibitors -- Evaluation of Drug-Transporter Interactions Using In Vitro Cell Models -- Plasma Protein-Binding Methods in Drug Discovery -- Isothermal Titration Calorimetry Characterization of Drug-Binding Energetics to Blood Proteins -- Metabolic Stability Assessed by Liver Microsomes and Hepatocytes -- In Vitro Drug Metabolite Profiling Using Hepatic S9 and Human Liver Microsomes -- In Vitro Identification of UDP-Glucuronosyltransferases (UGTs) Involved in Drug Metabolism -- In Vitro CYP Induction in Human Hepatocytes -- High-Throughput Screening of Human Cytochrome P450 Inhibitors Using Fluorometric Substrates -- Evaluation of Cytochrome P450 Inhibition in Human Liver Microsomes -- Identification of CYP Mechanism-Based Inhibitors -- Detection of DNA Adducts by 32P-Postlabeling Analysis -- Covalent DNA Adduct Formation Mediated by Cytochrome P450 -- Application of In Vitro Comet Assay for Genotoxicity Testing -- Assessing DNA Damage Using a Reporter Gene System -- Improvement of the Ames Test Using Human Liver S9 Preparation -- Screening for Chemical Mutagens Using the Mouse Lymphoma Assay -- A High-Throughput Binding Assay for HERG -- In Vitro Drug Metabolism -- In Vitro Screening Assay of the Reactivity of Acyl Glucuronides.
Keywords: Pharmacy; Pharmacy