Transgenic Plants Methods and Protocols - Peña, Leandro; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
Transgenic Plants: An Historical Perspective -- Transformation -- Plant Transformation: Agrobacterium-Mediated Gene Transfer -- Production of Hairy Root Cultures and Transgenic Plants by Agrobacterium rhizogenes-Mediated Transformation -- Stable Transformation of Plant Cells by Particle Bombardment/Biolistics -- Electroporation: Introduction and Expression of Transgenes in Plant Protoplasts -- Floral Dip: Agrobacterium-Mediated Germ Line Transformation -- Production of Transgenic Crops by the Floral-Dip Method -- Chloroplast Genetic Engineering to Improve Agronomic Traits -- Regeneration -- Organogenesis From Transformed Tomato Explants -- Genetic Transformation of Conifers Utilizing Somatic Embryogenesis -- Regeneration of Transgenic Cassava From Transformed Embryogenic Tissues -- Genetic Transformation of Mature Citrus Plants -- Selection -- Selectable Markers: Antibiotic and Herbicide Resistance -- Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection -- Green Fluorescent Protein Quantification in Whole Plants -- Positive Selection -- Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems -- Simple and Efficient Removal of Marker Genes From Plastids by Homologous Recombination -- Transgene Integration, Expression, and Localization -- The Study of Transgene Copy Number and Organization -- Analysis of Gene Expression in Transgenic Plants -- Transgene Integration: Use of Matrix Attachment Regions -- Fluorescence In Situ Hybridization to Localize Transgenes in Plant Chromosomes -- Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction -- Risk Assessment -- Agrobacterium Persistence in Plant Tissues After Transformation -- Transgene Dispersal Through Pollen -- Transgenic Crops -- Transgenic Crops: The Current and Next Generations -- Comparative Development and Impact of Transgenic Papayas in Hawaii, Jamaica, and Venezuela.
Keywords: Plant science; Botany; Transgenic organisms; Plant Sciences; Transgenics