Phosphodiesterase Methods and Protocols - Lugnier, Claire; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2005)
Study of Cyclic Adenosine Monophosphate Microdomains in Cells -- High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains -- Cygnets -- High-Throughput Screening of Phosphodiesterase Activity in Living Cells -- Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts -- Localization of the Cyclic Guanosine 3?,5?-Monophosphate- Hydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ Hybridization -- Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells -- Adenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3B -- Identification of Promoter Elements in the 5?-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase 3B Gene -- Purification of PDE6 Isozymes From Mammalian Retina -- Cyclic Guanosine 5?-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase -- Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies -- Determining the Subunit Structure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation -- Crystallization of Cyclic Nucleotide Phosphodiesterases -- Generation of PDE4 Knockout Mice by Gene Targeting -- Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase -- Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphorylation and Regulation of Activity of PDE4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases -- Radiolabeled Ligand Binding to the Catalytic or Allosteric Sites of PDE5 and PDE11 -- Analysis of Dimerization Determinants of PDE6 Catalytic Subunits -- Interactions Between Catalytic and Inhibitory Subunits of PDE6 -- Purification, Reconstitution on Lipid Vesicles, and Assays of PDE6 and Its Activator G Protein, Transducin.
Keywords: Biochemistry; Biochemistry, general