Molecular Toxicology Protocols - Keohavong, Phouthone; Grant, Stephen G.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2005)
Analysis of DNA Adducts -- 32P-Postlabeling Analysis of DNA Adducts -- Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot -- DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry -- Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpoles -- Analysis of DNA Strand Cleavage at Abasic Sites -- Detection of Chromosomal and Genome-Wide Damage -- Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probes -- Mutagen-Induced Chromatid Breakage as a Marker of Cancer Risk -- Flow Cytometric Analysis of Micronuclei in Erythrocytes -- The Comet Assay -- Computerized Image Analysis Software for the Comet Assay -- The Comet-FISH Technique -- DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis -- Detection and Characterization of Surrogate Gene Mutation -- Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes -- Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems -- Methods for Detecting Somatic Mutations In Vitro -- Molecular Analysis of Mutations in the Human HPRT Gene -- Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes -- The GPA In Vivo Somatic Mutation Assay -- Flow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR -- Detection and Characterization of Cancer Gene Mutation -- Mutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel Electrophoresis -- Analysis of K-RAS and P53 Mutations in Sputum Samples -- Allele-Specific Competitive Blocker-PCR Detection of Rare Base Substitution -- Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection -- Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions -- Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR -- Analysis of DNA Repair Mechanisms -- Microsatellite Instability -- Unscheduled DNA Synthesis -- Analysis of DNA Repair Using Transfection-Based Host Cell Reactivation -- An Immunoassay for Measuring Repair of Ultraviolet Photoproducts -- Analysis of DNA Double-Strand Break Repair by Nonhomologous End Joining in Cell-Free Extracts From Mammalian Cells -- Measuring Recombination Proficiency in Mouse Embryonic Stem Cells -- Array Technologies -- Strategies for Measurement of Biotransformation Enzyme Gene Expression -- Genotyping Technologies -- TaqMan® Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material -- Development of Quantitative Reverse Transcriptase PCR Assays for Measuring Gene Expression -- Apoptosis -- Quantification of Selective Phosphatidylserine Oxidation During Apoptosis -- Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance Spectroscopy and Annexin-Conjugated Iron -- Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay.
Keywords: Pharmacology; Biochemistry; Pharmacology/Toxicology.; Biochemistry, general