Ubiquitin-Proteasome Protocols - Patterson, Cam.; Cyr, Douglas M.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2005)
Biochemical Methods to Study the Ubiquitin-Proteasome System -- Small-Molecule Inhibitors of Proteasome Activity -- Purification of E1 and E1-Like Enzymes -- Assays for RING Family Ubiquitin Ligases -- Ubiquitin Chain Synthesis -- Purification of Proteasomes, Proteasome Subcomplexes, and Proteasome-Associated Proteins From Budding Yeast -- Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome -- Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradation -- Assays of Proteasome-Dependent Cleavage Products -- Identification of Components of Protein Complexes -- Mass Spectrometric Determination of Protein Ubiquitination -- Reconstitution of Endoplasmic Reticulum-Associated Degradation Using Yeast Membranes and Cytosol -- Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation -- Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization -- Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions -- Measuring Ubiquitin Conjugation in Cells -- Assays for Proteasome Assembly and Maturation -- N-Terminal Ubiquitination -- Quantitating Defective Ribosome Products -- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation -- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation -- Cystic Fibrosis Transmembrane Conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein Quality Control in Mammalian Cells -- Aggresome Formation -- Detection of Sumoylated Proteins -- Proteasome Inhibitors in Cancer Therapy -- Parkinson's Disease.
Keywords: Biochemistry; Biochemistry, general