Therapeutic Proteins Methods and Protocols - Smales, C. Mark.; James, David C.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2005)
Biopharmaceutical Proteins -- Expression of Antibody Fragments by Periplasmic Secretion in Escherichia coli -- Secretory Production of Therapeutic Proteins in Escherichia coli -- Expression of Recombinant LTB Protein in Marine vibrio VSP60 -- Heterologous Gene Expression in Yeast -- Pharmaceutical Proteins From Methylotrophic Yeasts -- Expression of Human Papillomavirus Type 16 L1 in Baculovirus Expression Systems -- Large-Scale Transient Expression of Therapeutic Proteins in Mammalian Cells -- Site-Specific Integration for High-Level Protein Production in Mammalian Cells -- Production of Recombinant Therapeutic Proteins by Mammalian Cells in Suspension Culture -- Transgene Control Engineering in Mammalian Cells -- Fusion to Albumin as a Means to Slow the Clearance of Small Therapeutic Proteins Using the Pichia pastoris Expression System -- High-Throughput Recovery of Therapeutic Proteins from the Inclusion Bodies of Escherichia coli -- Isolation, Solubilization, Refolding, and Chromatographic Purification of Human Growth Hormone from Inclusion Bodies of Escherichia coli Cells -- Large-Scale Preparation of Factor VIIa from Human Plasma -- Purification of Clinical-Grade Monoclonal Antibodies by Chromatographic Methods -- Virus Elimination and Validation -- Virus Removal by Nanofiltration -- Determining Residual Host Cell Antigen Levels in Purified Recombinant Proteins by Slot Blot and Scanning Laser Densitometry -- Principles of Biopharmaceutical Protein Formulation -- Solid-State Protein Formulation -- Stabilization of Therapeutic Proteins by Chemical and Physical Methods -- Extraction and Characterization of Vaccine Antigens from Water-in-Oil Adjuvant Formulations -- Probing Reversible Self-Association of Therapeutic Proteins by Sedimentation Velocity in the Analytical Ultracentrifuge -- Biological Characterization of Pegylated Interferons -- Characterization of Interferon ?2B Pegylated via Carboxyalkylation -- Quantifying Recombinant Proteins and Their Degradation Products Using SDS-PAGE and Scanning Laser Densitometry -- Extraction and Denaturing Gel Electrophoretic Methodology for the Analysis of Yeast Proteins -- Characterization of Therapeutic Proteins by Membrane and In-Gel Tryptic Digestion -- Oligosaccharide Release and MALDI-TOF MS Analysis of N-Linked Carbohydrate Structures from Glycoproteins -- Capillary Electrophoresis of Carbohydrates Derivatized With Fluorophoric Compounds -- High-Throughput LC/MS Methodology for ?(1?3)Gal Determination of Recombinant Monoclonal Antibodies -- Carbohydrate Structural Characterization of Fas Ligand Inhibitory Protein -- Top-Down Characterization of Protein Pharmaceuticals by Liquid Chromatography/Mass Spectrometry -- Sample Preparation Procedures for High-Resolution Nuclear Magnetic Resonance Studies of Aqueous and Stabilized Solutions of Therapeutic Peptides.
Keywords: Biochemistry; Biochemistry, general