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02596nam a22003375i 4500 |
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978-1-59259-958-5 |
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20191027041344.0 |
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cr nn 008mamaa |
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100301s2006 xxu| s |||| 0|eng d |
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|a 9781592599585
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7 |
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|a 10.1385/1592599583
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Yeast Protocols
|c edited by Wei Xiao.
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| 250 |
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|a 2nd ed. 2006.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2006.
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| 300 |
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|a XII, 392 p. 104 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 313
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|a Basic Investigations in Saccharomyces cerevisiae -- Isolation of Nucleic Acids -- Purification of Yeast Peroxisomes -- Isolation of Yeast Plasma Membranes -- Isolation of Yeast Mitochondria -- Extraction of Yeast Lipids -- Two-Dimensional Gel Electrophoresis of Total Yeast Proteins -- Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes -- Analysis of Yeast Lipids -- Yeast Fluorescence Microscopy -- Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe -- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method -- Mutagenesis -- Gene Disruption in the Budding Yeast Saccharomyces cerevisiae -- Inducible Degron and Its Application to Creating Conditional Mutants -- Synthetic Lethal Screen -- Synthetic Genetic Array Analysis in Saccharomyces cerevisiae -- Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates -- Chromatin Assembly in a Crude Fraction From Yeast Cells -- Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast -- Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning -- Study of Transcriptional Regulation Using a Reporter Gene Assay -- Assessing Telomeric Phenotypes -- Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast -- Production of Heterologous Proteins in Yeast With the Aid of the Hsp150? Carrier -- Yeast Two-Hybrid System Screening -- Import of Precursor Proteins Into Isolated Yeast Mitochondria.
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| 650 |
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|a Mycology.
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| 650 |
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|a Cell biology.
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| 650 |
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|a Biochemistry.
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| 650 |
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4 |
|a Mycology.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Biochemistry, general.
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| 700 |
1 |
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|a Xiao, Wei.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592599583
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