Yeast Protocols - Xiao, Wei.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2006)
Basic Investigations in Saccharomyces cerevisiae -- Isolation of Nucleic Acids -- Purification of Yeast Peroxisomes -- Isolation of Yeast Plasma Membranes -- Isolation of Yeast Mitochondria -- Extraction of Yeast Lipids -- Two-Dimensional Gel Electrophoresis of Total Yeast Proteins -- Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes -- Analysis of Yeast Lipids -- Yeast Fluorescence Microscopy -- Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe -- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method -- Mutagenesis -- Gene Disruption in the Budding Yeast Saccharomyces cerevisiae -- Inducible Degron and Its Application to Creating Conditional Mutants -- Synthetic Lethal Screen -- Synthetic Genetic Array Analysis in Saccharomyces cerevisiae -- Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates -- Chromatin Assembly in a Crude Fraction From Yeast Cells -- Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast -- Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning -- Study of Transcriptional Regulation Using a Reporter Gene Assay -- Assessing Telomeric Phenotypes -- Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast -- Production of Heterologous Proteins in Yeast With the Aid of the Hsp150? Carrier -- Yeast Two-Hybrid System Screening -- Import of Precursor Proteins Into Isolated Yeast Mitochondria.
Keywords: Mycology; Cell biology; Biochemistry; Mycology; Cell Biology; Biochemistry, general