Transmembrane Signaling Protocols - Ali, Hydar; Bodduluri, Haribabu; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2006)
Overviews -- Transmembrane Signaling by G Protein-Coupled Receptors -- Crosstalk Coregulation Mechanisms of G Protein- Coupled Receptors and Receptor Tyrosine Kinases -- Protein-Protein Interactions in Signaling Cascades -- Specific Topics -- Biological Role of the CXCR4-SDF-1 Axis in Normal Human Hematopoietic Cells -- Functional Expression of CXCR4 in Saccharomyces cerevisiae in the Development of Powerful Tools for the Pharmacological Characterization of CXCR4 -- Characterization of Constitutively Active Mutants of G Protein-Coupled Receptors -- G Protein-Coupled Receptor Dimerization and Signaling -- Real-Time Analysis of G Protein-Coupled Receptor Signaling in Live Cells -- Isolation of Membrane Rafts and Signaling Complexes -- Methods for the Study of Signaling Molecules in Membrane Lipid Rafts and Caveolae -- Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions -- Identification of Interacting Proteins Using the Yeast Two-Hybrid Screen -- Analysis of PDZ Domain Interactions Using Yeast Two-Hybrid and Coimmunoprecipitation Assays -- Clustering Assay for Studying the Interaction of Membrane Proteins with PDZ Domain Proteins -- Mammalian Cell Microinjection Assay to Study the Function of Rho Family Guanosine Triphosphatases -- Affinity-Based Assay of Rho Guanosine Triphosphatase Activation -- Assay of Phospholipase D Activity in Cell-Free Systems -- Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipase D -- Analysis of Global Gene Expression Profiles Activated by Chemoattractant Receptors -- Genetic Reconstitution of Bone Marrow for the Study of Signal Transduction Ex Vivo -- Proteomic Analysis of Human Neutrophils.
Keywords: Cell biology; Cell Biology