Plant Virology Protocols From Viral Sequence to Protein Function - Foster, Gary.; Johansen, Elisabeth; Hong, Yiguo; Nagy, Peter; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
General Introduction -- Plant-Virus Interactions -- Role of Capsid Proteins -- Role of Plant Virus Movement Proteins -- Multiple Roles of Viral Replication Proteins in Plant RNA Virus Replication -- Role of Silencing Suppressor Proteins -- Role of Vector-Transmission Proteins -- Viral Nucleic Acid Sequence/Function Analysis -- In Vivo Analyses of Viral RNA Translation -- In Vitro Analysis of Translation Enhancers -- Identification of Plant Virus IRES -- Analysis of Geminivirus DNA Replication by 2-D Gel -- Begomoviruses: Molecular Cloning and Identification of Replication Origin -- Analysis of Viroid Replication -- Biochemical Analyses of the Interactions Between Viral Polymerases and RNAs -- In Situ Detection of Plant Viruses and Virus-Specific Products -- Detection of siRNAs and miRNAs -- Cloning of Short Interfering RNAs from Virus-Infected Plants -- Solution Structure Probing of RNA Structures -- RNA Encapsidation Assay -- Protein Analysis and Investigation of Protein Function -- Surface Plasmon Resonance Analysis of Interactions Between Replicase Proteins of Tomato Bushy Stunt Virus -- Biochemical Approaches for Characterizing RNA-Protein Complexes in Preparation for High Resolution Structure Analysis -- Probing Interactions Between Plant Virus Movement Proteins and Nucleic Acids -- Movement Profiles: A Tool for Quantitative Analysis of Cell-to-Cell Movement of Plant Viral Movement Proteins -- Analysis of siRNA-Suppressor of Gene Silencing Interactions -- Phosphorylation Analysis of Plant Viral Proteins -- Analysis of Interactions Between Viral Replicase Proteins and Plant Intracellular Membranes -- Membrane and Protein Dynamics in Virus-Infected Plant Cells -- Site-Directed Mutagenesis of Whole Viral Genomes -- Viral Protein-Nucleic Acid Interaction: South (North)-Western Blot -- Protein-Protein Interactions: The Yeast Two-Hybrid System -- NMR Analysis of Viral Protein Structures -- Localization of Viral Proteins in Plant Cells: Protein Tagging -- Microscopy/GFP/Protein Tagging/Infections Clones and Other Such Tools -- Construction of Infectious Clones for RNA Viruses: TMV -- Construction of Infectious cDNA Clones for RNA Viruses: Turnip Crinkle Virus -- Construction of Infectious Clones for DNA Viruses: Mastreviruses -- Construction of Infectious Clones of Double-Stranded DNA Viruses of Plants Using Citrus Yellow Mosaic Virus as an Example -- Insertion of Introns: A Strategy to Facilitate Assembly of Infectious Full Length Clones -- Analysis of Cell-to-Cell and Long-Distance Movement of Apple Latent Spherical Virus in Infected Plants Using Green, Cyan, and Yellow Fluorescent Proteins -- Agroinoculation: A Simple Procedure for Systemic Infection of Plants with Viruses -- Geminivirus: Biolistic Inoculation and Molecular Diagnosis -- Genomics, Host Factors and Plant Based Studies -- Expression Microarrays in Plant-Virus Interaction -- Genome-Wide Screens for Identification of Host Factors in Viral Replication -- Phosphorylation of Movement Proteins by the Plasmodesmal-Associated Protein Kinase -- Virus-Induced Gene Silencing as a Tool to Identify Host Genes Affecting Viral Pathogenicity -- Yeast Two-Hybrid Assay to Identify Host-Virus Interactions.
Keywords: Plant science; Botany; Virology; Plant genetics; Plant Sciences; Virology; Plant Genetics and Genomics