Osteoporosis Methods and Protocols - Westendorf, Jennifer; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
In Vitro Models -- Isolation and Culture of Rodent Osteoprogenitor Cells -- Osteoclast Culture and Resorption Assays -- Assessing New Bone Formation in Neonatal Calvarial Organ Cultures -- Detection of Apoptosis of Bone Cells In Vitro -- In Vivo Rodent Models -- In Vivo Parathyroid Hormone Treatments and RNA Isolation and Analysis -- Assessment of Bone Formation Capacity Using In vivo Transplantation Assays: Procedure and Tissue Analysis -- Ossicle and Vossicle Implant Model Systems -- Utility of the Ovariectomized Rat as a Model for Human Osteoporosis in Drug Discovery -- Orchidectomy Models of Osteoporosis -- Methods of Gene Delivery -- Gene Delivery by Adenoviruses -- Lentivirus Delivery of shRNA Constructs into Osteoblasts -- Gene Delivery by Retroviruses -- Molecular and Genetic Techniques -- Chromatin Immunoprecipitation Assays: Application of ChIP-on-Chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells -- Identification of Transcription Factor Target Genes by ChIP Display -- Application of the Laser Capture Microdissection Technique for Molecular Definition of Skeletal Cell Differentiation In Vivo -- Quantitative Trait Loci Mapping -- Imaging Techniques -- In Situ Nuclear Organization of Regulatory Machinery -- Bioluminescent Imaging in Bone -- Micro-Computed Tomography: A Method for the Non-Destructive Evaluation of the Three-Dimensional Structure of Biological Specimens -- Fourier Transform-Infrared Microspectroscopy and Microscopic Imaging -- Mechanical Testing -- Assessment of the In Vivo Adaptive Response to Mechanical Loading -- Determination of Bone's Mechanical Matrix Properties by Nanoindentation -- Fluid Flow Assays.
Keywords: Orthopedics; Molecular biology; Cell biology; Radiology; Orthopedics; Molecular Medicine; Cell Biology; Imaging / Radiology