MicroRNA Protocols - Ying, Shao-Yao.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2006)
The MicroRNA: Overview of the RNA Gene That Modulates Gene Functions -- Structure Analysis of MicroRNA Precursors -- MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex -- Recognition and Cleavage of Primary MicroRNA Transcripts -- Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery -- MicroRNAs and Messenger RNA Turnover -- Prediction of MicroRNA Targets -- Prediction of Human MicroRNA Targets -- Complications in Mammalian MicroRNA Target Prediction -- miRBase:The MicroRNA Sequence Database -- Methodologies for High-Throughput Expression Profiling of MicroRNAs -- In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development -- Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA -- Cloning MicroRNAs From Mammalian Tissues -- Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation -- Identifying MicroRNA Regulators of Cell Death in Drosophila -- MicroRNAs in Human Immunodeficiency Virus-1 Infection -- Cloning and Detection of HIV-1-Encoded MicroRNA -- Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein -- In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates -- Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector -- Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs -- Isolation and Identification of Gene-Specific MicroRNAs -- Transgene-Like Animal Models Using Intronic MicroRNAs -- Evolution of MicroRNAs -- Perspectives.
Keywords: Cell biology; Cell Biology