Neural Stem Cells Methods and Protocols - Weiner, Leslie P.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Definitions and Criteria for Stem Cells -- Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard -- Isolation and Culture of NSCs -- Neural Differentiation of Human Embryonic Stem Cells -- Generating Neurons from Stem Cells -- Isolation of Neural Stem and Precursor Cells from Rodent Tissue -- Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization -- Adult Neural Stem Cells -- Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb -- Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium -- Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property -- Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood -- Production of Neurospheres from CNS Tissue -- Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells -- In Vitro Studies: A. Cellular Techniques -- Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures -- Telomere Neurobiology -- Chromosomal Mosaicism in Neural Stem Cells -- In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype -- In Vitro Migration Assays of Neural Stem Cells -- Measuring Apoptosis in Neural Stem Cells -- Markers of Adult Neural Stem Cells -- In Vitro Studies: B. Molecular Techniques -- Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR -- Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells -- Electroporation Strategies for Genetic Manipulation and Cell Labeling -- Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches -- Studies for Transplantation -- Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo -- Immunocytochemical Analysis of Neuronal Differentiation -- Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold -- Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS -- Optimizing Stem Cell Grafting into the CNS -- Routes of Stem Cell Administration in the Adult Rodent.
Keywords: Anthropology; Neurosciences; Cytology; Neurobiology; Anthropology; Neurosciences; Cell Biology; Neurobiology