YAC Protocols - MacKenzie, Alasdair; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2006)
Markers, Selection, and Media in Yeast Artificial Chromosome Cloning -- Construction of Yeast Artificial Chromosome Libraries From Pathogens and Nonmodel Organisms -- Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization -- Analysis of Yeast Artificial Chromosome DNA by Restriction Digestion, Southern Blotting Nucleic Acid Hybridization, and Polymerase Chain Reaction -- Postgenomic Bioinformatic Analysis of Yeast Artificial Chromosome Sequence -- Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes -- Modification and Amplification of Yeast Artificial Chromosomes -- Modifying Yeast Artificial Chromosomes to Generate Cre/LoxP and FLP/FRT Site-Specific Deletions and Inversions -- Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes -- A Polymerase Chain Reaction-Mediated Yeast Artificial Chromosome-Splitting Technology for Generating Targeted Yeast Artificial Chromosomes Subclones -- Genomic Reconstruction by Serial Mitotic Recombination of Yeast Artificial Chromosomes -- Targeting Vector Construction by Yeast Artificial Chromosome Modification -- Production of Yeast Artificial Chromosome Transgenic Mice by Pronuclear Injection of One-Cell Embryos -- Generation of Yeast Artificial Chromosome Transgenic Mice by Intracytoplasmic Sperm Injection -- Establishment of Cell Lines That Exhibit Correct Ontogenic Stage-Specific Gene Expression Profiles From Tissues of Yeast Artificial Chromosome Transgenic Mice Using Chemically Induced Growth Signals -- Application of Yeast Artificial Chromosomes in Fluorescence In Situ Hybridization -- Contribution of Yeast Artificial Chromosome-Based Physical Maps to the Final Assembly of the Trypanosoma cruzi Genome.
Keywords: Biochemistry; Human genetics; Biochemistry, general; Human Genetics