Prion Protein Protocols - Hill, Andrew F.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Cell Culture Models to Unravel Prion Protein Function and Aberrancies in Prion Diseases -- Investigation of PrPC Metabolism and Function in Live Cells -- Immunodetection of PrPSc Using Western and Slot Blotting Techniques -- Assaying Prions in Cell Culture -- Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes -- Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons -- Understanding the Nature of Prion Diseases Using Cell-free Assays -- Methods for Conversion of Prion Protein into Amyloid Fibrils -- Amplification of Purified Prions In Vitro -- Expression and Purification of Full-Length Recombinant PrP of High Purity -- Analysis of PrP Conformation Using Circular Dichroism -- Effect of Copper on the De Novo Generation of Prion Protein Expressed in Pichia pastoris -- Biophysical Investigations of the Prion Protein Using Electron Paramagnetic Resonance -- Molecular Diagnosis of Human Prion Disease -- Analysis of Endogenous PrPC Processing in Neuronal and Non-neuronal Cell Lines -- Molecular Typing of PrPres in Human Sporadic CJD Brain Tissue -- Transgenic Mouse Models of Prion Diseases -- Quantitative Bioassay of Surface-bound Prion Infectivity.
Keywords: Neurosciences; Proteins .; Biochemistry; Neurobiology; Infectious diseases; Cell biology; Neurosciences; Protein Science; Biochemistry, general; Neurobiology; Infectious Diseases; Cell Biology