Wnt Signaling Volume 1: Pathway Methods and Mammalian Models - Vincan, Elizabeth; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Wnt Signaling Pathway Methods -- The Canonical Wnt/?-Catenin Signalling Pathway -- Isolation and Application of Bioactive Wnt Proteins -- Purification and Wnt-Inhibitory Activities of Secreted Frizzled-Related Proteins -- Measuring GSK3 Expression and Activity in Cells -- Inhibition of Glycogen Synthase Kinase-3 -- Detection of Cytoplasmic and Nuclear Localization of Adenomatous Polyposis Coli (APC) Protein in Cells -- Detection of ?-Catenin Localization by Immunohistochemistry -- Assaying ?-Catenin/TCF Transcription with ?-Catenin/TCF Transcription-Based Reporter Constructs -- Native Promoter Reporters Validate Transcriptional Targets -- ?-Catenin-Independent Wnt Pathways: Signals, Core Proteins, and Effectors -- Image Analysis of Calcium Release Dynamics -- Detecting PKC Phosphorylation as Part of the Wnt/Calcium Pathway in Cutaneous Melanoma -- Measuring CamKII Activity in Xenopus Embryos as a Read-out for Non-canonical Wnt Signaling -- Analysis of Wnt7a-Stimulated JNK Activity and cJun Phosphorylation in Non-Small Cell Lung Cancer Cells -- ROCK Enzymatic Assay -- Detection of Planar Polarity Proteins in Mammalian Cochlea -- Mammalian Model Systems for Wnt/FZD Function -- Proteomic Analyses of Protein Complexes in the Wnt Pathway -- In Situ Hybridization to Evaluate the Expression of Wnt and Frizzled Genes in Mammalian Tissues -- Assaying Wnt5A-Mediated Invasion in Melanoma Cells -- Coculture Methodologies for the Study of Wnt Signals -- Analysis of Wnt/FZD-Mediated Signalling in a Cell Line Model of Colorectal Cancer Morphogenesis -- Analysing Tissue and Gene Function in Intestinal Organ Culture -- Genetics of Wnt Signaling During Early Mammalian Development -- Tissue-Specific Transgenic, Conditional Knockout and Knock-In Mice of Genes in the Canonical Wnt Signaling Pathway.
Keywords: Cell biology; Cell Biology