Protein Phosphatase Protocols - Moorhead, Greg.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2007)
Analysis of Protein Phosphatases -- A Brief Introduction to the Protein Phosphatase Families -- Small-Molecule Inhibitors of Ser/Thr Protein Phosphatases -- Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin-Sepharose and Microcystin-Biotin-Sepharose -- Utilizing Protein Phosphatase Inhibitors to Define PP2A as a Regulator of Ataxia-Telangiectasia Mutated -- An Automated Fluorescence-Based Method for Continuous Assay of PP2A Activity -- An In Vivo Assay to Quantify Stable Protein Phosphatase 2A (PP2A) Heterotrimeric Species -- Mutagenesis and Expression of the Scaffolding A? and A? Subunits of PP2A -- Isolation and Characterization of PP2A Holoenzymes Containing FLAG-Tagged B Subunits -- Purification of PP2A Holoenzymes by Sequential Immunoprecipitation with Anti-Peptide Antibodies -- Purification of PP2Ac from Bovine Heart -- Visualization of Intracellular PP1 Targeting Through Transiently and Stably Expressed Fluorescent Protein Fusions -- Yeast Two-Hybrid Screens to Identify Drosophila PP1-Binding Proteins -- Identification of Cellular Protein Phosphatase-1 Regulators -- Assay for Three-Way Interaction of Protein Phosphatase-1 (Glc7) With Regulatory Subunits Plus Phosphatase Inhibitor-2 -- Phosphorylation of the Protein Phosphatase Type 1 Inhibitor Protein CPI-17 by Protein Kinase C -- Purification of Smooth Muscle Myosin Phosphatase Using a Thiophosphorylated Myosin Light-Chain-Affinity Resin -- Proteins Interacting With Saccharomyces cerevisiae Type 1 Protein Phosphatase Catalytic Subunit Identified by Single-Step Affinity Purification and Mass Spectrometry -- Expression of Protein Histidine Phosphatase in Escherichia coli, Purification, and Determination of Enzyme Activity -- The Use of RNA Interference to Analyze Protein Phosphatase Function in Mammalian Cells -- Recognition of a PP2C Interaction Motif in Several Plant Protein Kinases -- Use of Yeast Genetic Tools to Define Biological Roles of Novel Protein Phosphatases -- Targeting of PP2C in Budding Yeast -- Phosphatase Targets in TOR Signaling -- Functional Characterization of Small CTD Phosphatases -- Genome-Scale Discovery and Characterization of Class-Specific Protein Sequences -- Yeast Substrate-Trapping System for Isolating Substrates of Protein Tyrosine Phosphatases.
Keywords: Biochemistry; Cell biology; Biochemistry, general; Cell Biology