Two-Dimensional Electrophoresis Protocols - Sheehan, David; Tyther, Raymond; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Two-Dimensional Electrophoresis: An Overview -- Solubilization of Proteins in 2DE: An Outline -- Selection of pH Ranges in 2DE -- Difficult Proteins -- Organelle Proteomics -- Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry -- Immunoblotting 2DE Membranes -- Troubleshooting Image Analysis in 2DE -- Analysis of Bacterial Proteins by 2DE -- Proteomic Analysis of Caenorhabditis elegans -- Protein Extraction for 2DE -- Analysis of Proteins from Marine Molluscs -- Preparation and Analysis of Plant and Plastid Proteomes by 2DE. -- High-Resolution 2DE -- Blue Native-Gel Electrophoresis Proteomics -- 2DE for Proteome Analysis of Human Metaphase Chromosomes -- Microsomal Proteomics -- Prefractionation Using Microscale Solution IEF -- Diagonal Electrophoresis for Detection of Protein Disulphide Bridges -- High-Resolution Large-Gel 2DE -- Silver Staining of Proteins in 2DE Gels -- Detection of 4-Hydroxy-2-Nonenal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach -- Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells -- Detection of Ubiquitination in 2DE -- Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry -- Detection of Protein Glutathionylation -- Activity-Based Protein Profiling of Protein Tyrosine Phosphatases -- Active Protease Mapping in 2DE Gels -- Two-Dimensional Difference Gel Electrophoresis -- Protein Expression Profiling -- C-Terminal Sequence Analysis of 2DE-Separated Proteins -- Shotgun Protein Analysis by Liquid Chromatography-Tandem Mass Spectrometry -- de Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination -- Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis -- Database Interrogation Algorithms for Identification of Proteins in Proteomic Separations -- Creating 2DE Databases for the World Wide Web.
Keywords: Biochemistry; Proteins .; Proteomics; Electrophoresis; Biochemistry, general; Protein Science; Proteomics; Electrophoresis