Microbial Gene Essentiality: Protocols and Bioinformatics - Osterman, Andrei L.; Gerdes, Svetlana Y.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Overview of Whole-Genome Essentiality Analysis -- Experimental Protocols -- Transposon-Based Strategies for the Identification of Essential Bacterial Genes -- Identification and Analysis of Essential Genes in Haemophilus influenzae -- Transposon Site Hybridization in Mycobacterium tuberculosis -- Essential Genes in the Infection Model of Pseudomonas aeruginosa PCR-Based Signature-Tagged Mutagenesis -- Whole-Genome Detection of Conditionally Essential and Dispensable Genes in Escherichia coli via Genetic Footprinting -- Generating a Collection of Insertion Mutations in the Staphylococcus aureus Genome Using bursa aurealis -- Multipurpose Transposon Insertion Libraries for Large-Scale Analysis of Gene Function in Yeast -- How to Make a Defined Near-Saturation Mutant Library. Case 1: Pseudomonas aeruginosa PAO1 -- Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality -- The Construction of Systematic In-Frame, Single-Gene Knockout Mutant Collection in Escherichia coli K-12 -- The Applications of Systematic In-Frame, Single-Gene Knockout Mutant Collection of Escherichia coli K-12 -- A Novel, Simple, High-Throughput Method for Isolation of Genome-Wide Transposon Insertion Mutants of Escherichia coli K-12 -- High-Throughput Creation of a Whole-Genome Collection of Yeast Knockout Strains -- Analysis of Genetic Interactions on a Genome-Wide Scale in Budding Yeast: Diploid-Based Synthetic Lethality Analysis by Microarray -- Scarless Engineering of the Escherichia coli Genome -- Minimization of the Escherichia coli Genome Using the Tn5-Targeted Cre/loxP Excision System -- Construction of Long Chromosomal Deletion Mutants of Escherichia coli and Minimization of the Genome -- Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library -- Techniques for the Isolation and Use of Conditionally Expressed Antisense RNA to Achieve Essential Gene Knockdowns in Staphylococcus aureus -- of Conditional Lethal Amber Mutations in Escherichia coli -- Bioinformatics -- Statistical Methods for Building Random Transposon Mutagenesis Libraries -- Statistical Evaluation of Genetic Footprinting Data -- Modeling Competitive Outgrowth of Mutant Populations: Why Do Essentiality Screens Yield Divergent Results? -- Statistical Analysis of Fitness Data Determined by TAG Hybridization on Microarrays -- Profiling of Escherichia coli Chromosome Database -- Gene Essentiality Analysis Based on DEG, a Database of Essential Genes -- Detection of Essential Genes in Streptococcus pneumoniae Using Bioinformatics and Allelic Replacement Mutagenesis -- Design and Application of Genome-Scale Reconstructed Metabolic Models -- Predicting Gene Essentiality Using Genome-Scale in Silico Models -- Comparative Approach to Analysis of Gene Essentiality.
Keywords: Biochemistry; Cell biology; Human genetics; Biochemistry, general; Cell Biology; Human Genetics