The Nucleus Volume 1: Nuclei and Subnuclear Components - Hancock, Ronald; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
The Intranuclear Environment -- The Intranuclear Environment -- Isolation of Nuclei -- Purification of Nuclei and Preparation of Nuclear Envelopes from Skeletal Muscle -- Isolation of Highly Purified Yeast Nuclei for Nuclease Mapping of Chromatin Structure -- Working with Oocyte Nuclei: Cytological Preparations of Active Chromatin and Nuclear Bodies from Amphibian Germinal Vesicles -- Preparation of Arabidopsis Nuclei and Nucleoli -- High-Yield Isolation and Subcellular Proteomic Characterization of Nuclear and Subnuclear Structures from Trypanosomes -- Methods for Studying the Nuclei and Chromosomes of Dinoflagellates -- The Nucleolus -- Isolation of Nucleoli from Ehrlich Ascites Tumor Cells and Dynamics of Nascent RNA within Isolated Nucleoli -- Time-lapse Microscopy and Fluorescence Resonance Energy Transfer to Analyze the Dynamics and Interactions of Nucleolar Proteins in Living Cells -- Three-Dimensional Reconstruction of Nucleolar Components by Electron Microscope Tomography -- Intranuclear Structures -- The Perinucleolar Compartment (PNC): Detection by Immunohistochemistry -- Isolation of the Constitutive Heterochromatin from Mouse Liver Nuclei -- Isolation of Pathology-Associated Intranuclear Inclusions -- The Nuclear Ubiquitin-Proteasome System: Visualization of Proteasomes, Protein Aggregates, and Proteolysis in the Cell Nucleus -- Interphase Chromosomes -- Multicolor 3D Fluorescence In Situ Hybridization for Imaging Interphase Chromosomes -- Fluorescent Transgenes to Study Interphase Chromosomes in Living Plants -- Analysis of Telomeres and Telomerase -- Combined Immunofluorescence, RNA Fluorescent In Situ Hybridization, and DNA Fluorescent In Situ Hybridization to Study Chromatin Changes, Transcriptional Activity, Nuclear Organization, and X-Chromosome Inactivation -- Analysis of the Mobility of DNA Double-Strand Break-Containing Chromosome Domains in Living Mammalian Cells.
Keywords: Biochemistry; Cell biology; Cell physiology; Biochemistry, general; Cell Biology; Cell Physiology